Singla Ramit, Jaitak Vikas
Centre for Pharmaceutical Sciences and Natural Products, School of Basic and Applied Sciences, Central University of Punjab, Bathinda(Pb) 151 001, India.
Centre for Pharmaceutical Sciences and Natural Products, School of Basic and Applied Sciences, Central University of Punjab, Bathinda(Pb) 151 001, India.
Phytochemistry. 2016 May;125:106-11. doi: 10.1016/j.phytochem.2016.03.004. Epub 2016 Mar 11.
Steviol glycosides (SG's) from Stevia rebaudiana (Bertoni) have been used as a natural low-calorie sweeteners. Its aftertaste bitterness restricts its use for human consumption and limits its application in food and pharmaceutical products. In present study, we have performed computational analysis in order to investigate the interaction of two major constituents of SG's against homology model of the hTAS2R4 receptor. Molecular simulation study was performed using stevioside and rebaudioside A revealed that, sugar moiety at the C-3'' position in rebaudioside A causes restriction of its entry into the receptor site thereby unable to trigger the bitter reception signaling cascade. Encouraged by the current finding, we have also developed a greener route using β-1,3-glucanase from Irpex lacteus for the synthesis of de-bittered rebaudioside A from stevioside. The rebaudioside A obtained was of high quality with percent conversion of 62.5%. The results here reported could be used for the synthesis of rebaudioside A which have large application in food and pharmaceutical industry.
甜叶菊（Stevia rebaudiana (Bertoni)）中的甜菊糖苷（SG's）已被用作天然低热量甜味剂。其回味苦味限制了其用于人类消费，并限制了其在食品和药品中的应用。在本研究中，我们进行了计算分析，以研究SG's的两种主要成分与hTAS2R4受体同源模型的相互作用。使用甜菊糖苷和莱鲍迪苷A进行的分子模拟研究表明，莱鲍迪苷A中C-3''位置的糖部分导致其进入受体位点受到限制，从而无法触发苦味接收信号级联反应。受当前发现的鼓舞，我们还开发了一条更环保的路线，使用来自白黄侧耳（Irpex lacteus）的β-1,3-葡聚糖酶从甜菊糖苷合成去苦的莱鲍迪苷A。得到的莱鲍迪苷A质量很高，转化率为62.5%。此处报道的结果可用于合成在食品和制药工业中有广泛应用的莱鲍迪苷A。
