Konovalov P V, Mitrofanova L B, Gorshkov A N, Ovsyannikov F A
North-Western Federal Medical Research Center, Saint Petersburg, Russia.
Arkh Patol. 2015 Sep-Oct;77(5):18-25. doi: 10.17116/patol201577518-25.
to reveal the morphological features of the lower uterine segment myometrium in connective tissue dysplasia (CTD) in women with uterine inertia.
Histological, immunohistochemical (with antibodies against collagen types I and III, matrix metalloproteinases 1 and 9 (MMR-1, MMP-9), tissue inhibitor of metalloproteinase 1 (TIMP-1), fibronectin; fibulin-5, connexin-43), electron microscopic, and electron immunocytochemical studies with morphometry of myometrial fragments from 15 parturient women with CTD and uterine inertia (a study group) and those from 10 women without CTD (a control group).
The myometrium in CTD exhibited the decreased expression of connextin-43, fibulin-5, TIMP-1, collagens types I and III with collagen type III predominance and the unchanged levels of fibronectin and MMP-1 and MMP-9. Electron microscopy and immunocytochemistry showed fewer intercellular contacts and the dramatically lower expression of connexin-43 than in the control.
A set of found myometrial changes in women with uterine inertia is a manifestation of CTD.
揭示宫缩乏力女性结缔组织发育异常（CTD）时子宫下段肌层的形态学特征。
对15例患有CTD且宫缩乏力的产妇（研究组）以及10例无CTD的女性（对照组）的子宫肌层碎片进行组织学、免疫组织化学（使用抗I型和III型胶原、基质金属蛋白酶1和9（MMP - 1、MMP - 9）、金属蛋白酶组织抑制剂1（TIMP - 1）、纤连蛋白；纤维连接蛋白 - 5、连接蛋白 - 43的抗体）、电子显微镜及电子免疫细胞化学研究，并进行形态计量分析。
CTD患者的子宫肌层中，连接蛋白 - 43、纤维连接蛋白 - 5、TIMP - 1、I型和III型胶原的表达降低，以III型胶原为主，纤连蛋白、MMP - 1和MMP - 9水平未改变。电子显微镜和免疫细胞化学显示，与对照组相比，细胞间连接减少，连接蛋白 - 43的表达显著降低。
宫缩乏力女性出现的一系列子宫肌层变化是CTD的一种表现。
