Imran Muhammad, Ashiq Muhammad Zar, Shafi Humera, Usman Hafiz Faisal, Wattoo Sardar Ali, Sarwar Muhammad, Tahir Muhammad Ashraf
Forensic Toxicology Department, Punjab Forensic Science Agency, Lahore 53700, Pakistan.
Forensic Toxicology Department, Punjab Forensic Science Agency, Lahore 53700, Pakistan.
Leg Med (Tokyo). 2016 Mar;19:5-10. doi: 10.1016/j.legalmed.2016.01.002. Epub 2016 Jan 6.
A dead body of middle aged man was exhumed from 6.5 month earth-grave. Autopsy findings were non-specific as the body was completely putrefied. Deceased's scalp hair and kidney was sent for toxicological analysis. Hair sample (50mg) was incubated with 1M NaOH (2 ml). Chloroquine was detected in hair and kidney during basic drug screen performed on GC/MS. For confirmation and quantitation, chloroquine was extracted using Hypersep verify CX SPE cartridges while mass detector was operated in SIM mode using the ions of m/z 245.0, 290.1, 319.0 for chloroquine while ions of m/z 260 and 455 were monitored for nalorphine (internal standard). Chloroquine was present in high concentration in hair (211 ng/mg) as well as in kidney (37.3mg/kg). Moreover, chloroquine was not detected in the wash solvents, suggesting ingestion of the drug rather than an external contamination of hair. These findings strongly suggested the acute exposure of higher doses of chloroquine to the deceased before death.
从一座埋葬了6.5个月的坟墓中挖出一具中年男性尸体。尸检结果不具特异性，因为尸体已完全腐烂。死者的头皮毛发和肾脏被送去做毒理学分析。将毛发样本（50毫克）与1M氢氧化钠（2毫升）一起孵育。在使用气相色谱/质谱仪进行的基础药物筛查中，在毛发和肾脏中检测到了氯喹。为了进行确认和定量，使用Hypersep verify CX固相萃取柱提取氯喹，同时质量检测器在选择离子监测模式下运行，使用氯喹的m/z 245.0、290.1、319.0离子，同时监测纳洛芬（内标）的m/z 260和455离子。氯喹在毛发中浓度很高（211纳克/毫克），在肾脏中也很高（37.3毫克/千克）。此外，在洗涤溶剂中未检测到氯喹，这表明是摄入了该药物而非毛发受到外部污染。这些发现有力地表明死者在死前曾急性接触高剂量氯喹。
