Chen Xinxin, Yang Jifei, Ji Yanhong, Okoth Edward, Liu Bin, Li Xiaoyang, Yin Hong, Zhu Qiyun
State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China.
International Livestock Research Institute, Nairobi, 00100, Kenya.
Virol Sin. 2016 Apr;31(2):150-9. doi: 10.1007/s12250-015-3692-2. Epub 2016 Mar 11.
African swine fever (ASF) is a lethal hemorrhagic disease that affects wild and domestic swine. The etiological agent of ASF is African swine fever virus (ASFV). Since the first case was described in Kenya in 1921, the disease has spread to many other countries. No commercial vaccines are available to prevent ASF. In this study, we generated a recombinant Newcastle disease virus (rNDV) expressing ASFV protein 72 (p72) by reverse genetics and evaluated its humoral and cellular immunogenicity in a mouse model. The recombinant virus, rNDV/p72, replicated well in embryonated chicken eggs and was safe to use in chicks and mice. The p72 gene in rNDV/p72 was stably maintained through ten passages. Mice immunized with rNDV/p72 developed high titers of ASFV p72 specific IgG antibody, and had higher levels of IgG1 than IgG2a. Immunization also elicited T-cell proliferation and secretion of IFN-γ and IL-4. Taken together, these results indicate that rNDV expressing ASFV p72 might be a potential vaccine candidate for preventing ASF.
非洲猪瘟（ASF）是一种影响野猪和家猪的致死性出血性疾病。ASF的病原体是非洲猪瘟病毒（ASFV）。自1921年在肯尼亚首次描述该病例以来，这种疾病已传播到许多其他国家。目前尚无用于预防ASF的商业疫苗。在本研究中，我们通过反向遗传学方法构建了一种表达ASFV蛋白72（p72）的重组新城疫病毒（rNDV），并在小鼠模型中评估了其体液免疫原性和细胞免疫原性。重组病毒rNDV/p72在鸡胚中复制良好，对雏鸡和小鼠使用安全。rNDV/p72中的p72基因在传代十次后仍能稳定维持。用rNDV/p72免疫的小鼠产生了高滴度的ASFV p72特异性IgG抗体，且IgG1水平高于IgG2a。免疫还引发了T细胞增殖以及IFN-γ和IL-4的分泌。综上所述，这些结果表明表达ASFV p72的rNDV可能是预防ASF的一种潜在疫苗候选物。
