Okamura S, Hayashi S, Kawasaki C, Kondo S, Asano Y, Omori F, Otsuka T, Shibuya T, Harada M, Niho Y
Nihon Ketsueki Gakkai Zasshi. 1989 Dec;52(8):1423-32.
A substantial number of leukemic blast colonies were formed when conditioned medium of human bladder carcinoma cell line 5637 was added as a stimulator. Recombinant colony-stimulating factor (CSF) also stimulated leukemic blast cell proliferation, leading to colony formation. Furthermore, serum CSF levels in some patients with acute myelogenous leukemia (AML), as detected by sensitive enzyme-linked immunosorbent assay (ELISA), were high. These observations prompted us to study further the expressions of hematopoietic growth factor genes. Granulocyte-macrophage CSF (GM-CSF) mRNA was detected in the leukemic blast cells from about 30% of patients with AML by Northern blot analysis using strict hybridization conditions with or without in vitro blast cell enrichment. These findings suggest that the expression of cytokine genes including the GM-CSF gene reflects in vivo phenomena, although no clear relationship between expression of the genes and serum CSF level has been established. Gene encoding tumor necrosis factor alpha (TNF-alpha), lymphotoxin (LT) and transforming growth factor beta (TGF-beta) were sometimes expressed in some malignant hematological cell lines and also some fresh leukemic cells.
当添加人膀胱癌细胞系5637的条件培养基作为刺激物时，形成了大量白血病原始细胞集落。重组集落刺激因子（CSF）也刺激白血病原始细胞增殖，导致集落形成。此外，通过灵敏的酶联免疫吸附测定（ELISA）检测发现，一些急性髓性白血病（AML）患者的血清CSF水平较高。这些观察结果促使我们进一步研究造血生长因子基因的表达。使用严格杂交条件，无论有无体外原始细胞富集，通过Northern印迹分析在约30%的AML患者的白血病原始细胞中检测到粒细胞-巨噬细胞CSF（GM-CSF）mRNA。这些发现表明，包括GM-CSF基因在内的细胞因子基因的表达反映了体内现象，尽管尚未确定基因表达与血清CSF水平之间的明确关系。编码肿瘤坏死因子α（TNF-α）、淋巴毒素（LT）和转化生长因子β（TGF-β）的基因有时在一些恶性血液学细胞系以及一些新鲜白血病细胞中表达。
