Signorelli Salvatore Santo, Volsi Guido Li, Pitruzzella Alessandro, Fiore Valerio, Mangiafico Marco, Vanella Luca, Parenti Rosalba, Rizzo Manfredi, Volti Giovanni Li
1 Department of Clinical and Experimental Medicine, University of Catania, Catania, Italy.
2 Biomedical and Biotechnological Science, University of Catania, Catania, Italy.
Angiology. 2016 Nov;67(10):945-950. doi: 10.1177/0003319716638242. Epub 2016 Jul 11.
Some emerging risk factors such as oxidative stress biomarkers and microRNAs (miRs) may add additional value to the established risk factors for peripheral artery disease (PAD). We enrolled 27 patients with PAD and 27 age-matched controls. We examined the levels of a series of miRs (miR-130a, miR-27b, and miR-210) in serum samples. The level of well-established oxidative stress biomarkers, such as lipid hydroperoxides, isoprostanes, hemeoxygenase-1 (HO-1) and reduced glutathione, was also measured in plasma and their relationship with the miRs was determined. Levels of miR-130a, miR-27b, and miR-210 were significantly increased in patients with PAD when compared to the controls. The level of miR-130 was positively correlated with body mass index, whereas miR-210 was inversely associated with pain-free walking distance (PfWD). None of the evaluated miRs was associated with lowered PfWD of patients with PAD (stage IIa > 250 m, IIb < 250 m) or oxidative stress parameters. In conclusion, our findings suggest the need for more research to assess if miRs can serve as useful markers for the early diagnosis and monitoring of PAD.
一些新兴的风险因素，如氧化应激生物标志物和微小RNA（miRs），可能为已确立的外周动脉疾病（PAD）风险因素增添额外价值。我们招募了27例PAD患者和27例年龄匹配的对照者。我们检测了血清样本中一系列miRs（miR-130a、miR-27b和miR-210）的水平。还测定了血浆中已确立的氧化应激生物标志物的水平，如脂质氢过氧化物、异前列腺素、血红素加氧酶-1（HO-1）和还原型谷胱甘肽，并确定了它们与miRs的关系。与对照组相比，PAD患者中miR-130a、miR-27b和miR-210的水平显著升高。miR-130的水平与体重指数呈正相关，而miR-210与无痛步行距离（PfWD）呈负相关。所评估的miRs均与PAD患者（IIa期>250 m，IIb期<250 m）降低的PfWD或氧化应激参数无关。总之，我们的研究结果表明需要进行更多研究，以评估miRs是否可作为PAD早期诊断和监测的有用标志物。
