Lipidomics Department, Institute of Cellular Biology and Pathology "Nicolae Simionescu" of the Romanian Academy, 8, B.P. Hasdeu Street, 050568 Bucharest, Romania.
Emergency Clinical Hospital "Prof. Dr. Agrippa Ionescu", 149 I.C. Brătianu Street, 077015 Baloteşti, Ilfov County, Romania.
Int J Mol Sci. 2020 Dec 16;21(24):9600. doi: 10.3390/ijms21249600.
There is an intensive effort to identify biomarkers to predict cardiovascular disease evolution. We aimed to determine the potential of microRNAs to predict the appearance of cardiovascular events (CVEs) in patients with peripheral artery disease (PAD) following femoral artery bypass surgery. Forty-seven PAD patients were enrolled and divided into two groups, without CVEs ( = 35) and with CVEs ( = 12), during 1 year follow-up. Intra-surgery atherosclerotic plaques from femoral arteries were collected and the levels of miR-142, miR-223, miR-155, and miR-92a of the primary transcripts of these microRNAs (pri-miRNAs), and gene expression of and were determined. Results showed that, in the plaques, miR-142, miR-223, and miR-155 expression levels were significantly increased in PAD patients with CVEs compared to those without CVEs. Positive correlations between these miRNAs and their pri-miRNAs levels and the expression were observed. In the plasma of PAD patients with CVEs compared to those without CVEs, miR-223 and miR-142 were significantly increased. The multiple linear regression analyses revealed significant associations among several plasma lipids, oxidative and inflammatory parameters, and plasma miRNAs levels. Receiver operator characteristic (ROC) analysis disclosed that plasma miR-142 levels could be an independent predictor for CVEs in PAD patients. Functional bioinformatics analyses supported the role of these miRNAs in the regulation of biological processes associated with atherosclerosis. Taken together, these data suggest that plasma levels of miR-142, miR-223, miR-155, and miR-92a can significantly predict CVEs among PAD patients with good accuracy, and that plasma levels of miR-142 can be an independent biomarker to predict post-surgery CVEs development in PAD patients.
目前正在积极努力寻找预测心血管疾病演变的生物标志物。本研究旨在确定 microRNAs 预测外周动脉疾病（PAD）患者股动脉旁路手术后出现心血管事件（CVE）的潜力。47 名 PAD 患者入选，并在 1 年随访期间分为无 CVE 组（n = 35）和有 CVE 组（n = 12）。收集股动脉术中动脉粥样硬化斑块，并测定这些 microRNAs 前体转录物（pri-miRNAs）的 miR-142、miR-223、miR-155 和 miR-92a 水平，以及 和 的基因表达。结果显示，与无 CVE 的 PAD 患者相比，有 CVE 的 PAD 患者斑块中的 miR-142、miR-223 和 miR-155 表达水平显著升高。观察到这些 miRNA 及其 pri-miRNAs 水平与 表达之间存在正相关。与无 CVE 的 PAD 患者相比，有 CVE 的 PAD 患者的血浆 miR-223 和 miR-142 显著升高。多元线性回归分析显示，几种血浆脂质、氧化和炎症参数与血浆 miRNAs 水平之间存在显著关联。受试者工作特征（ROC）分析显示，血浆 miR-142 水平可作为 PAD 患者 CVE 的独立预测因子。功能生物信息学分析支持这些 miRNA 在外周动脉疾病患者动脉粥样硬化相关生物学过程调控中的作用。总之，这些数据表明，miR-142、miR-223、miR-155 和 miR-92a 的血浆水平可以显著预测 PAD 患者的 CVE，并且血浆 miR-142 水平可以作为 PAD 患者术后 CVE 发展的独立生物标志物。
