Erenler Ramazan, Pabuccu Koksal, Yaglioglu Ayse Sahin, Demirtas Ibrahim, Gul Fatih
Z Naturforsch C J Biosci. 2016 Mar;71(3-4):87-92. doi: 10.1515/znc-2016-0010.
In this study, the effect of Mougeotia nummuloides and Spirulina major on Vero cells (African green monkey kidney), C6 cells (rat brain tumor cells) and HeLa cells (human uterus carcinoma) was investigated in vitro. The antiproliferative effect of the methanol extract of M. nummuloides and S. major compared with 5-fluorourasil (5-FU) and cisplatin was tested at various concentrations using the BrdU Cell Proliferation ELISA. Both M. nummuloides and S. major extracts significantly inhibited the proliferation of Vero, HeLa and C6 cancer cell lines with IC50 and IC75 values. The M. nummuloides extract exhibited higher activity than 5-FU and cisplatin on Vero and C6 cells at high concentrations. The S. major extract revealed better antifproliferative activity than standards against Vero cells at 500 μg/mL. The compounds of methanol extracts were determined by GC-MS after the silylation process. Trehalose, monostearin and 1-monopalmitin were detected as major products in the M. nummuloides extract where as in the S. major extract; monostearin, 1-monopalmitin and hexyl alcohol were the main constituents.
在本研究中，体外研究了钱币新月藻（Mougeotia nummuloides）和极大螺旋藻（Spirulina major）对Vero细胞（非洲绿猴肾细胞）、C6细胞（大鼠脑肿瘤细胞）和HeLa细胞（人子宫癌细胞）的影响。使用BrdU细胞增殖ELISA在不同浓度下测试了钱币新月藻和极大螺旋藻甲醇提取物与5-氟尿嘧啶（5-FU）和顺铂相比的抗增殖作用。钱币新月藻和极大螺旋藻提取物均显著抑制Vero、HeLa和C6癌细胞系的增殖，具有IC50和IC75值。钱币新月藻提取物在高浓度下对Vero和C6细胞表现出比5-FU和顺铂更高的活性。极大螺旋藻提取物在500μg/mL时对Vero细胞显示出比标准品更好的抗增殖活性。硅烷化处理后，通过GC-MS测定甲醇提取物的化合物。在钱币新月藻提取物中检测到海藻糖、单硬脂酸甘油酯和1-单棕榈酸甘油酯作为主要产物，而在极大螺旋藻提取物中，主要成分是单硬脂酸甘油酯、1-单棕榈酸甘油酯和己醇。
