巴戟天果实提取物的肿瘤细胞选择性抗增殖作用。

Tumor cell-selective antiproliferative effect of the extract from Morinda citrifolia fruits.

作者信息

Arpornsuwan Teerakul, Punjanon Tadsanee

机构信息

Faculty of Allied Health Science, Thammasat University, Pathumthani, Thailand.

出版信息

Phytother Res. 2006 Jun;20(6):515-7. doi: 10.1002/ptr.1902.

DOI:10.1002/ptr.1902

PMID:16619339

Abstract

The methanol extract from Morinda citrifolia fruits was tested for cytotoxicity activity on the MTT assay. The appearance of cytotoxic changes after exposure to the extract was in a concentration dependent manner. The median lethal concentrations (LC(50)) of the extract in baby hamster kidney (BHK) cells, African green monkey kidney (Vero) cells and human laryngeal carcinoma (Hep2) cells were found to be 2.5, 3 and 5 mg/mL, respectively. A concentration of 0.1 mg/mL of crude extract exhibited cytotoxic activity against breast cancer (MCF7) and neuroblastoma (LAN5) cell lines at 29% and 36%, respectively. The same concentration of extract showed no toxicity to Vero and very little toxicity to BHK (6%) and Hep2 (13%) cells.

摘要

对海巴戟果实的甲醇提取物进行了MTT法细胞毒性活性测试。暴露于提取物后细胞毒性变化的出现呈浓度依赖性。该提取物在幼仓鼠肾（BHK）细胞、非洲绿猴肾（Vero）细胞和人喉癌（Hep2）细胞中的半数致死浓度（LC(50)）分别为2.5、3和5 mg/mL。浓度为0.1 mg/mL的粗提取物对乳腺癌（MCF7）和神经母细胞瘤（LAN5）细胞系的细胞毒性活性分别为29%和36%。相同浓度的提取物对Vero细胞无毒性，对BHK细胞（6%）和Hep2细胞（13%）毒性很小。