Wang Xiaoyan, Ma Qifeng, Dou Lingling, Liu Zhen, Peng Renhai, Yu Shuxun
Anyang Institute of Technology, College of Biology and Food Engineering, Anyang, Henan, 455000, PR China.
State Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang, Henan, 455000, PR China.
Plant Physiol Biochem. 2016 Jun;103:106-19. doi: 10.1016/j.plaphy.2016.02.031. Epub 2016 Feb 27.
In plants, MLO (Mildew Locus O) gene encodes a plant-specific seven transmembrane (TM) domain protein involved in several cellular processes, including susceptibility to powdery mildew (PM). In this study, a genome-wide characterization of the MLO gene family in G. raimondii L., G. arboreum L. and G. hirsutum L. was performed. In total, 22, 17 and 38 homologous sequences were identified for each species, respectively. Gene organization, including chromosomal location, gene clustering and gene duplication, was investigated. Homologues related to PM susceptibility in upland cotton were inferred by phylogenetic relationships with functionally characterized MLO proteins. To conduct a comparative analysis between MLO candidate genes from G. raimondii L., G. arboreum L. and G. hirsutum L., orthologous relationships and conserved synteny blocks were constructed. The transcriptional variation of 38 GhMLO genes in response to exogenous application of salt, mannitol (Man), abscisic acid (ABA), ethylene (ETH), jasmonic acid (JA) and salicylic acid (SA) was monitored. Further studies should be conducted to elucidate the functions of MLO genes in PM susceptibility and phytohormone signalling pathways.
在植物中，MLO（白粉病位点O）基因编码一种植物特有的七跨膜（TM）结构域蛋白，参与多种细胞过程，包括对白粉病（PM）的易感性。在本研究中，对雷蒙德氏棉、亚洲棉和陆地棉中的MLO基因家族进行了全基因组特征分析。每个物种分别总共鉴定出22、17和38个同源序列。研究了基因组织，包括染色体定位、基因聚类和基因复制。通过与功能已明确的MLO蛋白的系统发育关系，推断出陆地棉中与白粉病易感性相关的同源物。为了对雷蒙德氏棉、亚洲棉和陆地棉的MLO候选基因进行比较分析，构建了直系同源关系和保守的共线性区段。监测了38个陆地棉MLO基因对外源施加盐、甘露醇（Man）、脱落酸（ABA）、乙烯（ETH）、茉莉酸（JA）和水杨酸（SA）的转录变化。应进一步开展研究以阐明MLO基因在白粉病易感性和植物激素信号通路中的功能。
