Vistarop Aldana G, Cohen Melina, De Matteo Elena, Preciado María Victoria, Chabay Paola A
Molecular Biology Laboratory, Pathology Division, Ricardo Gutiérrez Children's Hospital, Buenos Aires, Argentina.
Pathology Division, Ricardo Gutiérrez Children's Hospital, Buenos Aires, Argentina.
Sci Rep. 2016 Mar 18;6:23303. doi: 10.1038/srep23303.
Epstein-Barr virus (EBV) is a B lymphotropic human herpesvirus. Two models, germinal center (GC) and direct infection, describe how EBV infects B-cells. Since in Argentina primary infection is mostly subclinical at young ages, children represent an interesting population where to analyze EBV infection, especially considering that most studies are usually performed in adults. Tonsil biopsies from pediatric carriers were studied to describe infection characteristics. EBV+ lymphocytes at the interfollicular region were mainly observed. Latency III pattern in subepithelial (SubEp) lymphocytes was observed at young ages, probably indicating a recent infection. In older patients EBV was mostly detected in epithelial cells, suggesting that they could have been infected some time ago. This finding was sustained by tonsillar viral load, which was higher in cases with LMP1+SubEp cells vs. LMP1+nonSubEp cells (p = 0.0237, Mann-Whiney test). Latency III was prevalent and related to the GC, while latency II was associated with non-GC (p = 0.0159, χ2 test). EBERs+/IgD+ cells were statistically prevalent over EBERs+/CD27+ cells (p = 0.0021, χ2 test). These findings indicated that both EBV infection models are not mutually exclusive and provide some basis for further understanding of EBV infection dynamics. Moreover, we provide a more accurate explanation of EBV infection in pediatric asymptomatic carriers from a developing country.
爱泼斯坦-巴尔病毒（EBV）是一种嗜B淋巴细胞的人类疱疹病毒。生发中心（GC）和直接感染这两种模型描述了EBV如何感染B细胞。由于在阿根廷，原发性感染在年轻时大多为亚临床感染，儿童是分析EBV感染的一个有趣群体，尤其是考虑到大多数研究通常在成人中进行。对儿科携带者的扁桃体活检进行研究以描述感染特征。主要观察到滤泡间区域的EBV阳性淋巴细胞。在年轻时观察到上皮下（SubEp）淋巴细胞中的潜伏III型模式，这可能表明是近期感染。在老年患者中，EBV大多在上皮细胞中检测到，这表明他们可能在一段时间前就已被感染。这一发现得到了扁桃体病毒载量的支持，LMP1 + SubEp细胞病例的病毒载量高于LMP1 + 非SubEp细胞病例（p = 0.0237，曼-惠特尼检验）。潜伏III型普遍存在且与GC相关，而潜伏II型与非GC相关（p = 0.0159，χ²检验）。EBERs + /IgD + 细胞在统计学上比EBERs + /CD27 + 细胞更普遍（p = 0.0021，χ²检验）。这些发现表明，两种EBV感染模型并非相互排斥，为进一步了解EBV感染动态提供了一些依据。此外，我们对来自发展中国家的儿科无症状携带者的EBV感染提供了更准确的解释。
