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用于甾体化合物工业生物转化的雄烯二酮（AD）生产菌“新金色分枝杆菌”NRRL B - 3805的全基因组序列

Complete genome sequence of 'Mycobacterium neoaurum' NRRL B-3805, an androstenedione (AD) producer for industrial biotransformation of sterols.

作者信息

Rodríguez-García Antonio, Fernández-Alegre Estela, Morales Alejandro, Sola-Landa Alberto, Lorraine Jess, Macdonald Sandy, Dovbnya Dmitry, Smith Margaret C M, Donova Marina, Barreiro Carlos

机构信息

INBIOTEC (Instituto de Biotecnología de León), Parque Científico de León, Avda. Real 1, 24006 León, Spain; Área de Microbiología, Departamento de Biología Molecular, Universidad de León, Campus de Vegazana, 24071 León, Spain.

INBIOTEC (Instituto de Biotecnología de León), Parque Científico de León, Avda. Real 1, 24006 León, Spain.

出版信息

J Biotechnol. 2016 Apr 20;224:64-5. doi: 10.1016/j.jbiotec.2016.03.021. Epub 2016 Mar 14.

DOI:10.1016/j.jbiotec.2016.03.021
PMID:26988397
Abstract

Microbial bioconversion of sterols into high value steroid precursors, such as 4-androstene-3,17-dione (AD), is an industrial challenge. Genes and enzymes involved in sterol degradation have been proposed, although the complete pathway is not yet known. The genome sequencing of the AD producer strain 'Mycobacterium neoaurum' NRRL B-3805 (formerly Mycobacterium sp. NRRL B-3805) will serve to elucidate the critical steps for industrial processes and will provide the basis for further genetic engineering. The genome comprises a circular chromosome (5 421 338bp), is devoid of plasmids and contains 4844 protein-coding genes.

摘要

将甾醇微生物转化为高价值甾体前体，如4-雄烯-3,17-二酮（AD），是一项工业挑战。尽管完整途径尚不清楚，但已提出了参与甾醇降解的基因和酶。AD生产菌株“新金色分枝杆菌”NRRL B-3805（原分枝杆菌属NRRL B-3805）的基因组测序将有助于阐明工业过程的关键步骤，并将为进一步的基因工程提供基础。该基因组由一条环状染色体（5421338bp）组成，不含质粒，包含4844个蛋白质编码基因。

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