College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China.
Department of Surgery, University of Michigan Medical Center, Ann Arbor, MI 48109, United States.
Food Chem. 2016 Aug 1;204:167-175. doi: 10.1016/j.foodchem.2016.02.012. Epub 2016 Feb 12.
In this work, two flavonoid glycosides (neohesperidin and naringin) in lime fruit were effectively extracted by miniaturized matrix solid phase dispersion (MSPD), followed by ultra-performance liquid chromatography-ultraviolet detection. The best results were obtained using Florisil (150mg) as the sorbent and 1-butyl-3-methylimidazolium tetrafluoroborate (0.4mL, 250mM) as the elution solvent. This work represents the first attempt of using ionic liquids as a green eluent for extraction of the investigated compounds in miniaturized MSPD. Compared with the conventional methods, the proposed method is advantageous due to improved enrichment factor and reduced reagent consumption. A good linearity was observed with r(2) values (>0.998). Meanwhile, the method gave acceptable recoveries (90.16-96.47%) for the determination of flavonoids in plant samples. The limits of detection of the two analytes ranged between 4.08 and 5.04μg/g. The results showed that the optimized method has a great potential for sample preparation in routine analysis of complex plant samples.
在这项工作中，采用微型基质固相分散（MSPD）技术，有效地提取了酸橙果实中的两种类黄酮糖苷（新橙皮苷和柚皮苷），然后采用超高效液相色谱-紫外检测法进行分析。结果表明，最佳实验条件为：以弗罗里硅土（150mg）为吸附剂，1-丁基-3-甲基咪唑四氟硼酸盐（0.4mL，250mM）为洗脱溶剂。本研究首次尝试使用离子液体作为绿色洗脱剂，用于微型 MSPD 中目标化合物的提取。与传统方法相比，该方法具有提高的富集因子和减少的试剂消耗的优点。两种分析物的线性关系良好（r²值>0.998）。同时，该方法在植物样品中测定类黄酮时具有可接受的回收率（90.16-96.47%）。两种分析物的检出限范围在 4.08-5.04μg/g 之间。结果表明，该优化方法在复杂植物样品的常规分析中具有很大的样品制备潜力。
