Srivastava Kamna, Narang Rajiv, Bhatia Jagriti, Saluja Daman
Dr. B R Ambedkar Center for Biomedical Research, University of Delhi, Delhi-110007, India.
Department of Cardiology, All India Institute of Medical Sciences, New Delhi-110029, India.
PLoS One. 2016 Mar 18;11(3):e0151060. doi: 10.1371/journal.pone.0151060. eCollection 2016.
Hypertension is characterized by systemic high blood pressure and is the most common and important risk factor for the development of cardiovascular diseases. Studies have shown that the circulating levels of certain inflammatory markers such as tumor necrosis factor-alpha (TNF-alpha), interlukin-6 (IL-6), c-reactive protein (CRP), and tumor suppressor protein-53 (p53) are upregulated and are independently associated with essential hypertension. However, mechanism of increase in the levels of HSP70 protein is not clear. No such studies are reported in the blood circulation of patients with essential hypertension. In the present study, we investigated the expression of circulating HSP70 at mRNA and protein levels and its relationship with other inflammatory markers in patients with essential hypertension.
We recruited 132 patients with essential hypertension and 132 normal controls from similar socio-economic-geographical background. The expression of HSP70 at mRNA levels was determined by Real Time PCR and at protein levels by indirect Elisa and Western Blot techniques.
We found a significantly higher expression of HSP70 gene expression (approximately 6.45 fold, P < 0.0001) in hypertensive patients as compared to healthy controls. A significant difference (P < 0.0001) in the protein expression of HSP70 was also observed in plasma of patients as compared to that of controls.
Higher expression of HSP70 is positively correlated with inflammatory markers in patients with essential hypertension and this correlation could play an important role in essential hypertension.
高血压以全身性高血压为特征，是心血管疾病发生最常见且最重要的危险因素。研究表明，某些炎症标志物如肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）、C反应蛋白（CRP）和肿瘤抑制蛋白-53（p53）的循环水平上调，且与原发性高血压独立相关。然而，热休克蛋白70（HSP70）蛋白水平升高的机制尚不清楚。原发性高血压患者血液循环中尚无此类研究报道。在本研究中，我们调查了原发性高血压患者循环HSP70在mRNA和蛋白水平的表达及其与其他炎症标志物的关系。
我们从社会经济地理背景相似的人群中招募了132例原发性高血压患者和132名正常对照者。通过实时定量PCR测定HSP70在mRNA水平的表达，通过间接酶联免疫吸附测定法和蛋白质印迹技术测定其在蛋白水平的表达。
我们发现，与健康对照者相比，高血压患者中HSP70基因表达显著更高（约6.45倍，P < 0.0001）。与对照组相比，患者血浆中HSP70的蛋白表达也存在显著差异（P < 0.0001）。
原发性高血压患者中HSP70的高表达与炎症标志物呈正相关，这种相关性可能在原发性高血压中起重要作用。
