Du Jingjing, Xu Jianwei, Sun Zhenli, Jing Chuanyong
State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
Anal Chim Acta. 2016 Apr 7;915:81-9. doi: 10.1016/j.aca.2016.02.009. Epub 2016 Feb 12.
Several methods and materials have been explored for the sensitive and practicable detection of polycyclic aromatic hydrocarbons (PAHs). However, it is still a challenge to develop simple and cost-effective sensing techniques for PAHs. Herein we report the synthesis and construction of Fe3O4@Au SERS substrate. This magnetic substrate was composed by Fe3O4 microspheres and Au NPs. The size, morphology, and surface composition of Fe3O4@Au were characterized by multiple complimentary techniques including scanning electron microscopy, X-ray photoelectron spectroscopy, and X-ray powder diffraction. The spatial distributions of electro-magnetic field enhancement around Fe3O4@Au was calculated using finite difference time domain (FDTD) simulations. As a result of its remarkable sensitivity, the Fe3O4@Au-based SERS assay has been applied to detect the 16 EPA priority PAHs. The LODs achieved by our method (100-5 nM, 16.6-1.01 μg L(-1)) make it promising for the rapid screening of highly contaminated cases. As a proof-of-concept study, the substrate was applied in SERS sensing of PAHs in river matrix. The 16 PAHs could be differentiated based upon their characteristic SERS peaks. Most importantly, the detection was successfully conducted using a portable Raman spectrometer, which could be used for on-site monitoring of PAHs.
为了实现对多环芳烃（PAHs）的灵敏且实用的检测，人们探索了多种方法和材料。然而，开发用于PAHs的简单且经济高效的传感技术仍然是一项挑战。在此，我们报告了Fe3O4@Au表面增强拉曼散射（SERS）基底的合成与构建。这种磁性基底由Fe3O4微球和金纳米颗粒组成。通过多种互补技术对Fe3O4@Au的尺寸、形态和表面组成进行了表征，包括扫描电子显微镜、X射线光电子能谱和X射线粉末衍射。利用时域有限差分（FDTD）模拟计算了Fe3O4@Au周围电磁场增强的空间分布。由于其卓越的灵敏度，基于Fe3O4@Au的SERS检测方法已被应用于检测16种美国环境保护局（EPA）优先控制的PAHs。我们的方法所实现的检测限（100 - 5 nM，16.6 - 1.01 μg L(-1)）使其有望用于快速筛查高度污染的情况。作为一项概念验证研究，该基底被应用于河流基质中PAHs的SERS传感。这16种PAHs可以根据其特征SERS峰进行区分。最重要的是，使用便携式拉曼光谱仪成功进行了检测，该光谱仪可用于PAHs的现场监测。
