Department of Chemistry, University of Cincinnati, Cincinnati, OH, 45221, USA.
Mikrochim Acta. 2019 May 31;186(6):391. doi: 10.1007/s00604-019-3511-9.
A surface-enhanced Raman scattering (SERS) method is described for the determination of trace polycyclic aromatic hydrocarbons (PAHs) in the environment efficiently and economically. Detection sensitivity is improved by modifying gold nanoparticles (AuNPs) with 4-mercaptophenylboronic acid (4-MPBA) conjugated to β-cyclodextrin (β-CD) as a new method for ratiometric determination of PAHs in solution. Pyrene (with a Raman band at 580 cm) and anthracene (750 cm) were used as the model analytes, while 4-MPBA (1570 cm) was used as the internal reference to normalize the SERS signals. The intensity ratios of pyrene/4-MPBA increase linearly in the 2 to 10 nM pyrene concentration range, and the intensity ratios of anthracene/4-MPBA increase linearly in the 10 to 100 nM anthracene concentration range. The detection limits are 0.4 nM for pyrene and 4.4 nM for anthracene. This method was applied to the determination of the two analytes in soil sample extracts and the recoveries of pyrene (at levels of 236 ng∙g and 170 ng∙g) and anthracene (334 ng∙g and 510 ng∙g) agreed well with the results from GC-MS analyses. The good recovery rates (101.8% and 102.5% for pyrene and 106.4% and 101.7% for anthracene) confirmed the reliability of the method. Graphical abstract Schematic illustration of SERS signal enhancement of pyrene, as an example of polycyclic aromatic hydrocarbons, by β-cyclodextrin modified gold nanoparticles.
本文描述了一种表面增强拉曼散射（SERS）方法，用于高效、经济地测定环境中的痕量多环芳烃（PAHs）。通过用β-环糊精（β-CD）共轭的 4-巯基苯硼酸（4-MPBA）修饰金纳米粒子（AuNPs），作为溶液中 PAHs 比率测定的新方法，提高了检测灵敏度。以芘（拉曼带位于 580 cm）和蒽（750 cm）为模型分析物，而 4-MPBA（1570 cm）用作内标，以归一化 SERS 信号。在 2 至 10 nM 芘浓度范围内，芘/4-MPBA 的强度比呈线性增加，在 10 至 100 nM 蒽浓度范围内，蒽/4-MPBA 的强度比呈线性增加。检测限分别为 0.4 nM 芘和 4.4 nM 蒽。该方法应用于土壤样品提取物中两种分析物的测定，芘（236 ng∙g 和 170 ng∙g）和蒽（334 ng∙g 和 510 ng∙g）的回收率与 GC-MS 分析结果吻合良好。良好的回收率（芘为 101.8%和 102.5%，蒽为 106.4%和 101.7%）证实了该方法的可靠性。
