Peng Xiuming, Wu Haibo, Peng Xiaorong, Wu Xiaoxin, Cheng Linfang, Liu Fumin, Ji Shujing, Wu Nanping
State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310003, China.
Arch Virol. 2016 Jun;161(6):1665-70. doi: 10.1007/s00705-016-2826-7. Epub 2016 Mar 21.
Avian influenza viruses (AIVs) are known to cross species barriers, and emergent highly pathogenic H5N6 AIVs pose a serious threat to human health and the poultry industry. Here, we serially passaged an H5N6 virus 10 times in BALB/c mice. The pathogenicity of the wild-type 6D2 (WT-6D2) and mammal-adapted 6D2 strain (MA-6D2) were compared. The viral titer in multiple organs and the death rate for MA-6D2 were significantly higher than for WT-6D2. We provide evidence that the mutations HA A150V, NA R143K and G147E, PB2 E627K, and PA A343T may be important for adaptation of H5N6 AIVs to mammals.
已知禽流感病毒（AIVs）会跨越物种屏障，新出现的高致病性H5N6 AIVs对人类健康和家禽业构成严重威胁。在此，我们在BALB/c小鼠中对一株H5N6病毒连续传代10次。比较了野生型6D2（WT-6D2）和适应哺乳动物的6D2毒株（MA-6D2）的致病性。MA-6D2在多个器官中的病毒滴度和死亡率显著高于WT-6D2。我们提供的证据表明，HA A150V、NA R143K和G147E、PB2 E627K以及PA A343T这些突变可能对H5N6 AIVs适应哺乳动物很重要。
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