Garrido-Godino A I, García-López M C, García-Martínez J, Pelechano V, Medina D A, Pérez-Ortín J E, Navarro F
Departamento de Biología Experimental, Facultad de Ciencias Experimentales, Universidad de Jaén, Paraje de las Lagunillas, s/n, 23071 Jaén, Spain.
Departamento de Genética, Facultad de Biológicas, Universitat de València, Dr Moliner 50, E-46100 Burjassot, Valencia, Spain; ERI Biotecmed, Universitat de València, Dr Moliner 50, E-46100 Burjassot, Valencia, Spain.
Biochim Biophys Acta. 2016 May;1859(5):731-43. doi: 10.1016/j.bbagrm.2016.03.008. Epub 2016 Mar 18.
The RPB1 mutants in the foot region of RNA polymerase II affect the assembly of the complex by altering the correct association of both the Rpb6 and the Rpb4/7 dimer. Assembly defects alter both transcriptional activity as well as the amount of enzyme associated with genes. Here, we show that the global transcriptional analysis of foot mutants reveals the activation of an environmental stress response (ESR), which occurs at a permissive temperature under optimal growth conditions. Our data indicate that the ESR that occurs in foot mutants depends mostly on a global post-transcriptional regulation mechanism which, in turn, depends on Rpb4-mRNA imprinting. Under optimal growth conditions, we propose that Rpb4 serves as a key to globally modulate mRNA stability as well as to coordinate transcription and decay. Overall, our results imply that post-transcriptional regulation plays a major role in controlling the ESR at both the transcription and mRNA decay levels.
RNA聚合酶II足部区域的RPB1突变体通过改变Rpb6和Rpb4/7二聚体的正确缔合来影响复合物的组装。组装缺陷会改变转录活性以及与基因相关的酶的量。在这里，我们表明对足部突变体的全局转录分析揭示了环境应激反应（ESR）的激活，这种激活发生在最佳生长条件下的允许温度下。我们的数据表明，足部突变体中发生的ESR主要依赖于一种全局转录后调控机制，而这种机制又依赖于Rpb4-mRNA印记。在最佳生长条件下，我们提出Rpb4作为全局调节mRNA稳定性以及协调转录和衰变的关键。总体而言，我们的结果表明转录后调控在转录和mRNA衰变水平上控制ESR中起主要作用。
