Departamento de Biología Experimental-Genética, Universidad de Jaén, Paraje de las Lagunillas, s/n, E-23071 Jaén, Spain.
Genome Biology Unit, European Molecular Biology Laboratory (EMBL), Meyerhofstraße 1, 69117 Heidelberg, Germany.
Int J Mol Sci. 2023 Dec 29;25(1):507. doi: 10.3390/ijms25010507.
RNA pol II assembly occurs in the cytoplasm before translocation of the enzyme to the nucleus. Affecting this assembly influences mRNA transcription in the nucleus and mRNA decay in the cytoplasm. However, very little is known about the consequences on ncRNA synthesis. In this work, we show that impairment of RNA pol II assembly leads to a decrease in cryptic non-coding RNAs (preferentially CUTs and SUTs). This alteration is partially restored upon overcoming the assembly defect. Notably, this drop in ncRNAs is only partially dependent on the nuclear exosome, which suggests a major specific effect of enzyme assembly. Our data also point out a defect in transcription termination, which leads us to propose that CTD phosphatase Rtr1 could be involved in this process.
RNA pol II 组装发生在酶转运到细胞核之前的细胞质中。影响这种组装会影响细胞核中的 mRNA 转录和细胞质中的 mRNA 降解。然而，关于 ncRNA 合成的后果知之甚少。在这项工作中，我们表明，RNA pol II 组装的损伤导致隐匿性非编码 RNA（优先是 CUTs 和 SUTs）的减少。在克服组装缺陷后，这种改变部分得到恢复。值得注意的是，ncRNAs 的下降部分仅依赖于核 exosome，这表明酶组装具有主要的特异性影响。我们的数据还指出转录终止存在缺陷，这使我们提出 CTD 磷酸酶 Rtr1 可能参与这一过程。
Zotero 插件
