Saptalena Lena Ganda, Kuklya Andriy, Telgheder Ursula
Faculty of Chemistry, Instrumental Analytical Chemistry, University of Duisburg-Essen, Universitätsstr. 5, 45141, Essen, Germany.
IWW Water Centre, Moritzstr. 26, 45476, Mülheim a.d. Ruhr, Germany.
Anal Bioanal Chem. 2016 May;408(14):3715-25. doi: 10.1007/s00216-016-9456-5. Epub 2016 Mar 22.
In this study, we demonstrate that the combination of an enzymatic method (based on Colilert-18 medium) and gas chromatography-differential mobility spectrometry (GC-DMS) can reduce the time required for detection of coliform bacteria (including Escherichia coli) from 18 to 2.5 h. The presented method includes the incubation (~2.5 h) of the sample containing coliform bacteria in Colilert-18 medium. The incubation time of 2.5 h is required for the activation of the β-galactosidase enzyme. Produced during the incubation biomarker o-nitrophenol (ONP) can be detected by means of GC-DMS within just 200 s. The detection limit for ONP was 45 ng (on-column). The method developed in this work provides significantly shorter analysis time compared with standard methods, and can be potentially adapted to the field conditions. Therefore, this method is a promising tool for an early detection of coliform bacteria (including E. coli). Graphical Abstract Fast detection of coliform bacteria by means of GC-DMS.
在本研究中，我们证明酶法（基于Colilert - 18培养基）与气相色谱 - 差分迁移谱法（GC - DMS）相结合可将检测大肠菌群（包括大肠杆菌）所需的时间从18小时缩短至2.5小时。所提出的方法包括将含有大肠菌群的样品在Colilert - 18培养基中孵育（约2.5小时）。激活β - 半乳糖苷酶需要2.5小时的孵育时间。孵育过程中产生的生物标志物邻硝基苯酚（ONP）可在短短200秒内通过GC - DMS检测到。ONP的检测限为45纳克（柱上）。与标准方法相比，本研究开发的方法显著缩短了分析时间，并且有可能适用于现场条件。因此，该方法是早期检测大肠菌群（包括大肠杆菌）的一种有前景的工具。图形摘要 通过GC - DMS快速检测大肠菌群
