Dumesic Daniel A, Guedikian Annie A, Madrigal Vanessa K, Phan Julia D, Hill David L, Alvarez Juan P, Chazenbalk Gregorio D
Department of Obstetrics and Gynecology (D.A.D., A.A.G., V.K.M., J.D.P., J.P.A., G.D.C., University of California, Los Angeles, Los Angeles, California 90095; ART Reproductive Center (D.L.H.), Beverly Hills, California 90210.
J Clin Endocrinol Metab. 2016 May;101(5):2235-45. doi: 10.1210/jc.2016-1464. Epub 2016 Mar 22.
Complex cumulus cell-oocyte interactions govern energy utilization during oocyte development.
This study investigates the relationship of cumulus cell mitochondria with oocyte development during ovarian stimulation for in vitro fertilization (IVF).
This is a prospective cohort study.
The setting was an academic center.
Thirty women underwent ovarian stimulation for IVF.
INTERVENTION(S): Pooled cumulus cells were collected; numbers of total and mature oocytes and two-pronuclear (day 1), six- to eight-cell cleavage (day 3), and blastocyst (day 5) embryos were recorded.
MAIN OUTCOME MEASURE(S): A mitochondrial bioassay was developed with Jurkat cells and used with cumulus cells from IVF patients to correlate mitochondrial membrane potential resistance to carbonyl cyanide 3-chlorophenylhydrazone (CCCP) stress with oocyte development and embryogenesis.
Adjusting for FSH administered and maternal age, cumulus cell mitochondrial membrane potential resistance to CCCP positively correlated with numbers of total (P < .025) and mature (P < .025) oocytes retrieved. The highest oocyte numbers that correlated with cumulus cell mitochondrial membrane potential occurred in women with the greatest ovarian response to FSH (mitochondrial membrane potential resistance to CCCP-log FSH interactions: total oocytes P < .025; mature oocytes P < .05). Multiple regression modeling of mature oocyte numbers, age, and cumulus cell mitochondrial membrane potential resistance to CCCP showed that numbers of mature oocytes best correlated with numbers of embryos at all stages (P < .0001).
During ovarian stimulation for IVF, cumulus cell mitochondrial membrane potential resistance to stress correlates with numbers of total and mature oocytes retrieved, suggesting that cumulus cell-oocyte interactions involving energy facilitate oocyte development.
复杂的卵丘细胞-卵母细胞相互作用控制着卵母细胞发育过程中的能量利用。
本研究调查在体外受精（IVF）的卵巢刺激过程中，卵丘细胞线粒体与卵母细胞发育之间的关系。
这是一项前瞻性队列研究。
学术中心。
30名接受IVF卵巢刺激的女性。
收集合并的卵丘细胞；记录总的和成熟的卵母细胞数量以及双原核（第1天）、6至8细胞期分裂（第3天）和囊胚（第5天）胚胎的数量。
用Jurkat细胞开发了一种线粒体生物测定法，并将其用于IVF患者的卵丘细胞，以将线粒体膜电位对羰基氰化物3-氯苯腙（CCCP）应激的抗性与卵母细胞发育和胚胎发生相关联。
校正促卵泡激素（FSH）给药量和母亲年龄后，卵丘细胞线粒体膜电位对CCCP的抗性与回收的总卵母细胞数量（P <.025）和成熟卵母细胞数量（P <.025）呈正相关。与卵丘细胞线粒体膜电位相关的最高卵母细胞数量出现在对FSH卵巢反应最大的女性中（线粒体膜电位对CCCP的抗性与FSH的相互作用：总卵母细胞P <.025；成熟卵母细胞P <.05）。对成熟卵母细胞数量、年龄和卵丘细胞线粒体膜电位对CCCP的抗性进行多元回归建模显示，成熟卵母细胞数量与所有阶段的胚胎数量相关性最好（P <.0001）。
在IVF的卵巢刺激过程中，卵丘细胞线粒体膜电位对应激的抗性与回收 的总卵母细胞和成熟卵母细胞数量相关，这表明涉及能量的卵丘细胞-卵母细胞相互作用促进了卵母细胞发育。
