Yamazaki W, Uemura R, Sekiguchi S, Dong J-B, Watanabe S, Kirino Y, Mekata H, Nonaka N, Norimine J, Sueyoshi M, Goto Y, Horii Y, Kurogi M, Yoshino S, Misawa N
Center for Animal Disease Control, University of Miyazaki, Miyazaki, Japan.
Miyazaki Prefectural Institute for Public Health and Environment, Miyazaki, Japan.
J Appl Microbiol. 2016 Jun;120(6):1711-22. doi: 10.1111/jam.13141. Epub 2016 Apr 25.
To elucidate the distribution and circulation dynamics of Campylobacter and Salmonella in Japanese chicken broiler flocks.
A 2-year investigation of the distribution of Campylobacter and Salmonella was conducted in 25 broiler flocks at nine farms in Japan from 2013 to 2014. Campylobacter and Salmonella tested positive in 11 (44·0%) and 24 (96·0%) broiler flocks respectively. One hundred and ninety-five Campylobacter and 184 Salmonella isolates were characterized into 12 Campylobacter (including two novel genotypes) and three Salmonella MLST genotypes. Only Salmonella isolation between caecal and environmental samples were significantly correlated. Further, one litter sample tested positive for Salmonella before new chicks were introduced. The Campylobacter strains rapidly lost culturability within 2-18 days; in contrast, the Salmonella strains survived from 64-211 days in artificially inoculated water samples.
No persistent circulation-mediated Campylobacter contamination was observed. In contrast, circulation of Salmonella in broiler houses was seen, apparently due to the litter excreted from broiler flocks, as well as Salmonella-contaminated water and feed.
This paper provides the distribution, genotypic data and circulation dynamics of Campylobacter and Salmonella as recently observed in Japanese chicken broiler farms.
阐明弯曲杆菌和沙门氏菌在日本肉鸡群中的分布及循环动态。
2013年至2014年，对日本9个农场的25个肉鸡群进行了为期2年的弯曲杆菌和沙门氏菌分布调查。弯曲杆菌和沙门氏菌分别在11个（44.0%）和24个（96.0%）肉鸡群中检测呈阳性。195株弯曲杆菌和184株沙门氏菌分离株被鉴定为12种弯曲杆菌（包括两种新基因型）和3种沙门氏菌多位点序列分型基因型。仅盲肠和环境样本之间的沙门氏菌分离存在显著相关性。此外，在引入新雏鸡之前，一份垫料样本检测出沙门氏菌呈阳性。弯曲杆菌菌株在2 - 18天内迅速丧失可培养性；相比之下，沙门氏菌菌株在人工接种的水样中存活64 - 211天。
未观察到由持续循环介导的弯曲杆菌污染。相比之下，在肉鸡舍中发现了沙门氏菌的循环，显然是由于肉鸡群排出的垫料以及受沙门氏菌污染的水和饲料。
本文提供了日本肉鸡养殖场中最近观察到的弯曲杆菌和沙门氏菌的分布、基因型数据及循环动态。
