Liu Zhongtian, Zhou Gang, Ren Chonghua, Xu Kun, Yan Qiang, Li Xinyi, Zhang Tingting, Zhang Zhiying
College of Animal Science & Technology, Shaan'xi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling, Shaan'xi 712100, China.
Research Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi 030006, China.
Vaccine. 2016 Apr 29;34(20):2378-82. doi: 10.1016/j.vaccine.2016.03.036. Epub 2016 Mar 20.
Yeast is considered as a simple and cost-effective host for protein expression, and our previous studies have proved that Saccharomyces cerevisiae can deliver recombinant protein and DNA into mouse dendritic cells and can further induce immune responses as novel vaccines. In order to know whether similar immune responses can be induced in rabbit by oral administration of such recombinant S. cerevisiae vaccine, we orally fed the rabbits with heat-inactivated myostatin-recombinant S. cerevisiae for 5 weeks, and then myostatin-specific antibody in serum was detected successfully by western blotting and ELISA assay. The rabbits treated with myostatin-recombinant S. cerevisiae vaccine grew faster and their muscles were much heavier than that of the control group. As a common experimental animal and a meat livestock with great economic value, rabbit was proved to be the second animal species that have been successfully orally immunized by recombinant S. cerevisiae vaccine after mice.
酵母被认为是一种用于蛋白质表达的简单且经济高效的宿主，我们之前的研究已经证明，酿酒酵母可以将重组蛋白和DNA递送至小鼠树突状细胞，并能够作为新型疫苗进一步诱导免疫反应。为了了解口服这种重组酿酒酵母疫苗是否能在兔体内诱导类似的免疫反应，我们用热灭活的肌肉生长抑制素重组酿酒酵母对兔进行了5周的口服投喂，然后通过蛋白质印迹法和酶联免疫吸附测定成功检测到血清中的肌肉生长抑制素特异性抗体。用肌肉生长抑制素重组酿酒酵母疫苗处理的兔生长更快，其肌肉比对照组重得多。兔作为一种常见的实验动物和具有巨大经济价值的肉用家畜，被证明是继小鼠之后第二种成功通过重组酿酒酵母疫苗进行口服免疫的动物物种。
