• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

最佳的γ疱疹病毒适应性在体外和体内都需要多个裂解性复制起点。

Multiple Lytic Origins of Replication Are Required for Optimal Gammaherpesvirus Fitness In Vitro and In Vivo.

作者信息

Sattler Christine, Steer Beatrix, Adler Heiko

机构信息

Research Unit Gene Vectors, Helmholtz Zentrum München - German Research Center for Environmental Health (GmbH), Munich, Germany.

出版信息

PLoS Pathog. 2016 Mar 23;12(3):e1005510. doi: 10.1371/journal.ppat.1005510. eCollection 2016 Mar.

DOI:10.1371/journal.ppat.1005510
PMID:27007137
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4805163/
Abstract

An unresolved question in herpesvirus biology is why some herpesviruses contain more than one lytic origin of replication (oriLyt). Using murine gammaherpesvirus 68 (MHV-68) as model virus containing two oriLyts, we demonstrate that loss of either of the two oriLyts was well tolerated in some situations but not in others both in vitro and in vivo. This was related to the cell type, the organ or the route of inoculation. Depending on the cell type, different cellular proteins, for example Hexim1 and Rbbp4, were found to be associated with oriLyt DNA. Overexpression or downregulation of these proteins differentially affected the growth of mutants lacking either the left or the right oriLyt. Thus, multiple oriLyts are required to ensure optimal fitness in different cell types and tissues.

摘要

疱疹病毒生物学中一个尚未解决的问题是，为何有些疱疹病毒含有不止一个裂解性复制起点（oriLyt）。我们以含有两个oriLyt的鼠γ疱疹病毒68（MHV-68）作为模型病毒，证明在某些情况下，两个oriLyt中的任何一个缺失在体外和体内都能被很好地耐受，但在其他情况下则不然。这与细胞类型、器官或接种途径有关。根据细胞类型的不同，发现不同的细胞蛋白，如Hexim1和Rbbp4，与oriLyt DNA相关联。这些蛋白的过表达或下调对缺失左或右oriLyt的突变体的生长有不同影响。因此，需要多个oriLyt来确保在不同细胞类型和组织中的最佳适应性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a45b/4805163/eb4bd189b6f2/ppat.1005510.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a45b/4805163/353093593347/ppat.1005510.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a45b/4805163/961e1a555d4e/ppat.1005510.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a45b/4805163/a50c4bf32971/ppat.1005510.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a45b/4805163/8e7417374cee/ppat.1005510.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a45b/4805163/dcb9ec7b03bd/ppat.1005510.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a45b/4805163/51b125ba4ae2/ppat.1005510.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a45b/4805163/4f0bff0c4ffe/ppat.1005510.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a45b/4805163/eb4bd189b6f2/ppat.1005510.g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a45b/4805163/353093593347/ppat.1005510.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a45b/4805163/961e1a555d4e/ppat.1005510.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a45b/4805163/a50c4bf32971/ppat.1005510.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a45b/4805163/8e7417374cee/ppat.1005510.g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a45b/4805163/dcb9ec7b03bd/ppat.1005510.g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a45b/4805163/51b125ba4ae2/ppat.1005510.g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a45b/4805163/4f0bff0c4ffe/ppat.1005510.g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a45b/4805163/eb4bd189b6f2/ppat.1005510.g008.jpg

相似文献

1
Multiple Lytic Origins of Replication Are Required for Optimal Gammaherpesvirus Fitness In Vitro and In Vivo.最佳的γ疱疹病毒适应性在体外和体内都需要多个裂解性复制起点。
PLoS Pathog. 2016 Mar 23;12(3):e1005510. doi: 10.1371/journal.ppat.1005510. eCollection 2016 Mar.
2
Identification of cis sequences required for lytic DNA replication and packaging of murine gammaherpesvirus 68.鉴定小鼠γ疱疹病毒68裂解性DNA复制和包装所需的顺式序列。
J Virol. 2004 Sep;78(17):9123-31. doi: 10.1128/JVI.78.17.9123-9131.2004.
3
CCAAT/enhancer binding proteins play a role in oriLyt-dependent genome replication during MHV-68 de novo infection.CCAAT/增强子结合蛋白在 MHV-68 从头感染期间 oriLyt 依赖性基因组复制中发挥作用。
Protein Cell. 2011 Jun;2(6):463-9. doi: 10.1007/s13238-011-1060-z. Epub 2011 Jul 12.
4
Identification and functional characterization of the left origin of lytic replication of murine gammaherpesvirus 68.小鼠γ-疱疹病毒68裂解复制左起始位点的鉴定及功能特性分析
Virology. 2009 May 10;387(2):285-95. doi: 10.1016/j.virol.2009.02.029. Epub 2009 Mar 12.
5
The M10 locus of murine gammaherpesvirus 68 contributes to both the lytic and the latent phases of infection.鼠γ疱疹病毒68的M10基因座对感染的裂解期和潜伏期均有影响。
J Virol. 2009 Aug;83(16):8163-72. doi: 10.1128/JVI.00629-09. Epub 2009 Jun 3.
6
Murine Gammaherpesvirus 68 ORF48 Is an RTA-Responsive Gene Product and Functions in both Viral Lytic Replication and Latency during In Vivo Infection.小鼠γ-疱疹病毒68的ORF48是一种RTA反应性基因产物，在体内感染期间在病毒裂解复制和潜伏中均发挥作用。
J Virol. 2015 Jun;89(11):5788-800. doi: 10.1128/JVI.00406-15. Epub 2015 Mar 11.
7
Murine gammaherpesvirus 68 contains two functional lytic origins of replication.鼠γ-疱疹病毒68含有两个功能性裂解复制起点。
J Virol. 2007 Jul;81(13):7300-5. doi: 10.1128/JVI.02406-06. Epub 2007 Apr 18.
8
Unconventional sequence requirement for viral late gene core promoters of murine gammaherpesvirus 68.非常规序列要求：鼠γ疱疹病毒 68 的病毒晚期基因核心启动子。
J Virol. 2014 Mar;88(6):3411-22. doi: 10.1128/JVI.01374-13. Epub 2014 Jan 8.
9
Murine gammaherpesvirus 68 open reading frame 11 encodes a nonessential virion component.小鼠γ-疱疹病毒68开放阅读框11编码一种非必需的病毒体成分。
J Virol. 2005 Mar;79(5):3163-8. doi: 10.1128/JVI.79.5.3163-3168.2005.
10
Dynamic association of gammaherpesvirus DNA with core histone during de novo lytic infection of primary cells.在原代细胞的从头裂解性感染过程中，γ疱疹病毒 DNA 与核心组蛋白的动态关联。
Virology. 2011 Dec 20;421(2):167-72. doi: 10.1016/j.virol.2011.09.024. Epub 2011 Oct 20.

引用本文的文献

1
KSHV genome harbors both constitutive and lytically induced enhancers.卡波西肉瘤相关疱疹病毒基因组包含组成型和裂解诱导型增强子。
J Virol. 2024 Jun 13;98(6):e0017924. doi: 10.1128/jvi.00179-24. Epub 2024 May 2.
2
Variation within major internal repeats of KSHV in vivo.卡波西肉瘤相关疱疹病毒（KSHV）体内主要内部重复序列的变异
Virus Evol. 2023 May 22;9(1):vead034. doi: 10.1093/ve/vead034. eCollection 2023.
3
Epigenetic Reprogramming of Kaposi's Sarcoma-Associated Herpesvirus during Hypoxic Reactivation.缺氧再激活过程中卡波西肉瘤相关疱疹病毒的表观遗传重编程

本文引用的文献

1
Gene essentiality and synthetic lethality in haploid human cells.单倍体人细胞中的基因必需性和合成致死性。
Science. 2015 Nov 27;350(6264):1092-6. doi: 10.1126/science.aac7557. Epub 2015 Oct 15.
2
Inhibition of cdk9 during herpes simplex virus 1 infection impedes viral transcription.单纯疱疹病毒1型感染期间对细胞周期蛋白依赖性激酶9的抑制会阻碍病毒转录。
PLoS One. 2013 Oct 18;8(10):e79007. doi: 10.1371/journal.pone.0079007. eCollection 2013.
3
Antimicrobial effects of murine mesenchymal stromal cells directed against Toxoplasma gondii and Neospora caninum: role of immunity-related GTPases (IRGs) and guanylate-binding proteins (GBPs).
Cancers (Basel). 2022 Nov 2;14(21):5396. doi: 10.3390/cancers14215396.
4
Conquering the Host: Determinants of Pathogenesis Learned from Murine Gammaherpesvirus 68.征服宿主：从鼠γ疱疹病毒 68 中学到的发病机制决定因素。
Annu Rev Virol. 2021 Sep 29;8(1):349-371. doi: 10.1146/annurev-virology-011921-082615.
5
RNA-guided gene editing of the murine gammaherpesvirus 68 genome reduces infectious virus production.RNA 引导的基因编辑可减少小鼠γ疱疹病毒 68 基因组的传染性病毒产生。
PLoS One. 2021 Jun 4;16(6):e0252313. doi: 10.1371/journal.pone.0252313. eCollection 2021.
6
The DNase Activity of Kaposi's Sarcoma-Associated Herpesvirus SOX Protein Serves an Important Role in Viral Genome Processing during Lytic Replication.卡波西肉瘤相关疱疹病毒 SOX 蛋白的 DNA 酶活性在裂解复制过程中对病毒基因组加工起着重要作用。
J Virol. 2019 Apr 3;93(8). doi: 10.1128/JVI.01983-18. Print 2019 Apr 15.
7
Tick-Borne Transmission of Murine Gammaherpesvirus 68.鼠γ疱疹病毒 68 通过蜱虫传播。
Front Cell Infect Microbiol. 2017 Oct 31;7:458. doi: 10.3389/fcimb.2017.00458. eCollection 2017.
8
Reactivation and Lytic Replication of Kaposi's Sarcoma-Associated Herpesvirus: An Update.卡波西肉瘤相关疱疹病毒的再激活与裂解性复制：最新进展
Front Microbiol. 2017 Apr 20;8:613. doi: 10.3389/fmicb.2017.00613. eCollection 2017.
9
RTA Occupancy of the Origin of Lytic Replication during Murine Gammaherpesvirus 68 Reactivation from B Cell Latency.在小鼠γ-疱疹病毒68从B细胞潜伏期重新激活期间，裂解复制起始位点的RTA占据情况
Pathogens. 2017 Feb 16;6(1):9. doi: 10.3390/pathogens6010009.
鼠间质基质细胞对弓形虫和新孢子虫的抗菌作用：免疫相关 GTP 酶（IRGs）和鸟嘌呤核苷酸结合蛋白（GBPs）的作用。
Med Microbiol Immunol. 2013 Jun;202(3):197-206. doi: 10.1007/s00430-012-0281-y. Epub 2012 Dec 27.
4
Potent antiviral activity of topoisomerase I and II inhibitors against Kaposi's sarcoma-associated herpesvirus.拓扑异构酶 I 和 II 抑制剂对卡波西肉瘤相关疱疹病毒的强大抗病毒活性。
Antimicrob Agents Chemother. 2012 Feb;56(2):893-902. doi: 10.1128/AAC.05274-11. Epub 2011 Nov 21.
5
Pathogenesis and host control of gammaherpesviruses: lessons from the mouse.γ疱疹病毒的发病机制与宿主控制：来自小鼠的启示。
Annu Rev Immunol. 2011;29:351-97. doi: 10.1146/annurev-immunol-072710-081639.
6
Initiation of Epstein-Barr virus lytic replication requires transcription and the formation of a stable RNA-DNA hybrid molecule at OriLyt.启动 Epstein-Barr 病毒裂解复制需要转录，并在 OriLyt 形成稳定的 RNA-DNA 杂交分子。
J Virol. 2011 Mar;85(6):2837-50. doi: 10.1128/JVI.02175-10. Epub 2010 Dec 29.
7
Inhibition of T-cell proliferation by murine multipotent mesenchymal stromal cells is mediated by CD39 expression and adenosine generation.鼠源多能间充质基质细胞通过 CD39 表达和腺苷生成抑制 T 细胞增殖。
Cell Transplant. 2011;20(8):1221-30. doi: 10.3727/096368910X546553. Epub 2010 Nov 5.
8
Human cytomegalovirus UL29/28 protein interacts with components of the NuRD complex which promote accumulation of immediate-early RNA.人巨细胞病毒 UL29/28 蛋白与 NuRD 复合物的成分相互作用，促进即刻早期 RNA 的积累。
PLoS Pathog. 2010 Jun 24;6(6):e1000965. doi: 10.1371/journal.ppat.1000965.
9
The M10 locus of murine gammaherpesvirus 68 contributes to both the lytic and the latent phases of infection.鼠γ疱疹病毒68的M10基因座对感染的裂解期和潜伏期均有影响。
J Virol. 2009 Aug;83(16):8163-72. doi: 10.1128/JVI.00629-09. Epub 2009 Jun 3.
10
Identification and functional characterization of the left origin of lytic replication of murine gammaherpesvirus 68.小鼠γ-疱疹病毒68裂解复制左起始位点的鉴定及功能特性分析
Virology. 2009 May 10;387(2):285-95. doi: 10.1016/j.virol.2009.02.029. Epub 2009 Mar 12.