Silva Francine Maria de França, Santos Edna Michely de Sá, Torres Sandra Maria, Yamasak Elise Miyuki, Ramos Rafael Antonio Nascimento, Alves Leucio Câmara
Departamento de Medicina Veterinária, Universidade Federal Rural de Pernambuco, Recife, PE, Brasil.
Unidade Acadêmica de Garanhuns, Universidade Federal Rural de Pernambuco, Garanhuns, PE, Brasil.
Rev Bras Parasitol Vet. 2016 Jan-Mar;25(1):127-30. doi: 10.1590/S1984-29612016014. Epub 2016 Mar 18.
The skin is the site of inoculation of Leishmania spp. in susceptible hosts, and consequently dermatopathies, especially ulcerative dermatitis, are the main clinical signs observed. The aim of this study was to assess parasitism of the skin (intact and ulcerated) among dogs that were naturally infected by Leishmania spp., through immunohistochemical analysis. Skin fragments (intact and ulcerated) were collected from 13 dogs with positive parasitological (bone marrow aspiration and exfoliative skin) and serological examinations (ELISA S7 Biogene) for Leishmania spp. These samples were processed using the immunohistochemical technique, involving the streptavidin-peroxidase complex. Ulcerative lesions were mainly observed on the elbows (53.84%; 7/13), nostrils (15.38%; 2/13), ears (23.07%; 3/13) and wings of the ilium (7.69%; 1/13). A severe parasite load was detected in 46.15% and 76.92% of the intact and ulcerated skin samples tested, respectively. The parasite load on ulcerated skin was statistically higher than on intact skin (p = 0.0221). These results indicate that the intact and ulcerated skin may host a high parasite load of amastigote forms of Leishmania spp., which can favor the transmission of the parasite.
皮肤是利什曼原虫属在易感宿主中的接种部位，因此皮肤病，尤其是溃疡性皮炎，是观察到的主要临床症状。本研究的目的是通过免疫组织化学分析，评估自然感染利什曼原虫属的犬类皮肤（完整和溃疡）中的寄生虫感染情况。从13只经寄生虫学（骨髓穿刺和皮肤脱落细胞检查）和血清学检查（ELISA S7 Biogene）确诊为利什曼原虫属阳性的犬类身上采集皮肤碎片（完整和溃疡）。这些样本采用免疫组织化学技术处理，涉及链霉亲和素-过氧化物酶复合物。溃疡性病变主要出现在肘部（53.84%；7/13）、鼻孔（15.38%；2/13）、耳朵（23.07%；3/13）和髂骨翼（7.69%；1/13）。在检测的完整皮肤样本和溃疡皮肤样本中，分别有46.15%和76.92%检测到严重的寄生虫负荷。溃疡皮肤的寄生虫负荷在统计学上高于完整皮肤（p = 0.0221）。这些结果表明，完整和溃疡的皮肤可能携带大量利什曼原虫属无鞭毛体形式的寄生虫负荷，这可能有利于寄生虫的传播。
