Hacisuleyman Ezgi, Shukla Chinmay J, Weiner Catherine L, Rinn John L
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
Department of Stem Cell and Regenerative Biology, Harvard University7 Divinity Avenue, Room 305, Cambridge, Massachusetts 02138, USA.
Nat Commun. 2016 Mar 24;7:11021. doi: 10.1038/ncomms11021.
More than half the human and mouse genomes are comprised of repetitive sequences, such as transposable elements (TEs), which have been implicated in many biological processes. In contrast, much less is known about other repeats, such as local repeats that occur in multiple instances within a given locus in the genome but not elsewhere. Here, we systematically characterize local repeats in the genomic locus of the Firre long noncoding RNA (lncRNA). We find a conserved function for the RRD repeat as a ribonucleic nuclear retention signal that is sufficient to retain an otherwise cytoplasmic mRNA in the nucleus. We also identified a repeat, termed R0, that can function as a DNA enhancer element within the intronic sequences of Firre. Collectively, our data suggest that local repeats can have diverse functionalities and molecular modalities in the Firre locus and perhaps more globally in other lncRNAs.
超过一半的人类和小鼠基因组由重复序列组成，如转座元件（TEs），这些序列与许多生物学过程有关。相比之下，对于其他重复序列，如在基因组中给定基因座内多次出现但在其他地方不存在的局部重复序列，人们了解得要少得多。在这里，我们系统地表征了Firre长链非编码RNA（lncRNA）基因组位点中的局部重复序列。我们发现RRD重复序列具有作为核糖核酸核保留信号的保守功能，该信号足以将原本位于细胞质的mRNA保留在细胞核中。我们还鉴定出一种名为R0的重复序列，它可以在Firre的内含子序列中作为DNA增强子元件发挥作用。总体而言，我们的数据表明局部重复序列在Firre基因座中可能具有多种功能和分子模式，或许在其他lncRNA中也是如此。
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