Belair David G, Le Ngoc Nhi, Murphy William L
Department of Biomedical Engineering, University of Wisconsin-Madison, USA.
Biomater Sci. 2016 May 26;4(5):819-25. doi: 10.1039/c5bm00633c. Epub 2016 Mar 24.
Platelets contain an abundance of growth factors that mimic the composition of the wound healing milieu, and platelet-derived VEGF in particular can negatively influence wound healing if unregulated. Here, we sought to capture and regulate the activity of VEGF factor from human platelets using poly(ethylene glycol) microspheres. In this communication, we demonstrate that platelet freeze/thaw produced significantly higher levels of Vascular Endothelial Growth Factor (VEGF) than either calcium chloride treatment, protease activated receptor 1 activating peptide (PAR1AP) treatment, or thrombin treatment. PEG microspheres containing a VEGF-binding peptide (VBP), derived from VEGFR2, sequestered VEGF from platelet concentrate, prepared via freeze/thaw, and reduced the bioactivity of platelet concentrate in HUVEC culture, which suggests that VBP microspheres sequestered and reduced the activity of VEGF from patient-derived platelets. Here, we demonstrate the ability of VEGF sequestering microspheres to regulate the activity of VEGF derived from a growth factor-rich autologous human blood product.
血小板含有大量模拟伤口愈合环境成分的生长因子，特别是血小板衍生的血管内皮生长因子（VEGF），如果不受调控，会对伤口愈合产生负面影响。在此，我们试图使用聚乙二醇微球捕获并调控来自人血小板的VEGF因子的活性。在本通讯中，我们证明血小板冻融产生的血管内皮生长因子（VEGF）水平显著高于氯化钙处理、蛋白酶激活受体1激活肽（PAR1AP）处理或凝血酶处理。含有源自VEGFR2的VEGF结合肽（VBP）的聚乙二醇微球，从通过冻融制备的血小板浓缩物中隔离VEGF，并降低了血小板浓缩物在人脐静脉内皮细胞（HUVEC）培养中的生物活性，这表明VBP微球隔离并降低了来自患者源血小板的VEGF的活性。在此，我们展示了VEGF隔离微球调控源自富含生长因子的自体人血制品的VEGF活性的能力。
