Freeman Lindsay M, Smolyaninov Alexei, Pang Lin, Fainman Yeshaiahu
Department of Electrical and Computer Engineering, University of California San Diego, 9500 Gilman Drive, La Jolla, California 92093-0407, USA.
Sci Rep. 2016 Mar 24;6:23535. doi: 10.1038/srep23535.
Plasmonic devices are of great interest due to their ability to confine light to the nanoscale level and dramatically increase the intensity of the electromagnetic field, functioning as high performance platforms for Raman signal enhancement. While Raman spectroscopy has been proposed as a tool to identify the preferential binding sites and adsorption configurations of molecules to nanoparticles, the results have been limited by the assumption that a single binding site is responsible for molecular adsorption. Here, we develop the simulated Raman correlation spectroscopy (SRCS) process to determine which binding sites of a molecule preferentially bind to a plasmonic material and in what capacity. We apply the method to the case of nucleic acids binding to silver, discovering that multiple atoms are responsible for adsorption kinetics. This method can be applied to future systems, such as to study the molecular orientation of adsorbates to films or protein conformation upon adsorption.
等离子体设备因其能够将光限制在纳米尺度并显著增强电磁场强度而备受关注，可作为拉曼信号增强的高性能平台。虽然拉曼光谱已被提议作为一种识别分子与纳米颗粒的优先结合位点和吸附构型的工具，但结果受到单一结合位点负责分子吸附这一假设的限制。在此，我们开发了模拟拉曼相关光谱（SRCS）方法，以确定分子的哪些结合位点优先与等离子体材料结合以及以何种方式结合。我们将该方法应用于核酸与银结合的情况，发现多个原子负责吸附动力学。该方法可应用于未来的系统，例如研究吸附物在薄膜上的分子取向或吸附时的蛋白质构象。
