Wang Yi, Wu Lin, Wong Ten It, Bauch Martin, Zhang Qingwen, Zhang Jinling, Liu Xiaohu, Zhou Xiaodong, Bai Ping, Dostalek Jakub, Liedberg Bo
Centre for Biomimetic Sensor Science, School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Drive, Singapore 637553.
Electronics and Photonics Department, Institute of High Performance Computing, Agency for Science, Technology, and Research (A*STAR), 1 Fusionopolis Way, Singapore 138632.
Nanoscale. 2016 Apr 21;8(15):8008-16. doi: 10.1039/c5nr08816j.
We investigated the simultaneous excitation of localized surface plasmons (LSPs) and propagating surface plasmons (PSPs) on a thin metallic film with an array of nanoholes for the enhancement of fluorescence intensity in heterogeneous bioassays. Experiments supported by simulations reveal that the co-excitation of PSP and LSP modes on the nanohole array in a Kretschmann configuration allows for fluorescence enhancement of about 10(2) as compared to a flat Au surface irradiated off-resonance. Moreover, this fluorescence signal was about 3-fold higher on the substrate supporting both PSPs and LSPs than that on a flat surface where only PSPs were resonantly excited. Simulations also indicated the highly directional fluorescence emission as well as the high fluorescence collection efficiency on the nanohole array substrate. Our contribution attempts to de-convolute the origin of this enhancement and identify further ways to maximize the efficiency of surface plasmon-enhanced fluorescence spectroscopy for implementation in ultra-sensitive bioassays.
我们研究了在具有纳米孔阵列的薄金属膜上同时激发局域表面等离子体（LSP）和传播表面等离子体（PSP），以增强异质生物测定中的荧光强度。模拟支持的实验表明，在Kretschmann配置下，纳米孔阵列上PSP和LSP模式的共同激发与非共振照射的平坦金表面相比，可使荧光增强约10²。此外，在同时支持PSP和LSP的基底上，该荧光信号比仅PSP被共振激发的平坦表面上的荧光信号高约3倍。模拟还表明，纳米孔阵列基底上具有高度定向的荧光发射以及高荧光收集效率。我们的贡献旨在解开这种增强的起源，并确定进一步提高表面等离子体增强荧光光谱效率的方法，以用于超灵敏生物测定。
