Yang Xin, Yang Jiang, Wang Hongning, Li Caiwu, He Yongguo, Jin SenYan, Zhang Hemin, Li Desheng, Wang Pengyan, Xu Yuesong, Xu Changwen, Fan Chengyun, Xu Lulai, Huang Shan, Qu Chunmao, Li Guo
J Zoo Wildl Med. 2016 Mar;47(1):374-8. doi: 10.1638/2015-0203.1.
In order to study the typical vaginal bacterial flora of giant pandas (Ailuropoda melanoleuca), we took vaginal swabs for the sake of bacterial isolation, from 24 healthy female giant pandas. A total of 203 isolates were identified, representing a total of 17 bacterial species. The most common bacteria isolated were Lactobacillus spp. (54.2%, 13 of 24), followed by Staphylococcus epidermidis (41.7%, 10 of 24) and Escherichia coli (33.3%, 8 of 24). Some opportunistic pathogenic bacteria, such as Peptostreptococcus spp., Klebsiella pneumoniae, and Proteus mirabilis, were also isolated but showed no pathology. Antimicrobial susceptibility testing of aerobic bacterial isolates was performed with disk diffusion method. Of the 152 isolates, resistance was most frequently observed with chloramphenicol (17.8%), followed by tetracycline (14.5%), ciprofloxacin (12.5%), streptomycin (11.8%), and florfenicol (11.8%), while 7.2% were multidrug resistant. This is the first report of the normal vaginal culturable bacterial flora of giant pandas, followed by the antimicrobial susceptibility patterns of the isolates.
为了研究大熊猫（Ailuropoda melanoleuca）的典型阴道菌群，我们从24只健康雌性大熊猫身上采集阴道拭子用于细菌分离。共鉴定出203株分离菌，代表17种细菌。分离出的最常见细菌是乳酸杆菌属（54.2%，24只中的13只），其次是表皮葡萄球菌（41.7%，24只中的10只）和大肠杆菌（33.3%，24只中的8只）。还分离出一些机会致病菌，如消化链球菌属、肺炎克雷伯菌和奇异变形杆菌，但未显示出病理学特征。采用纸片扩散法对需氧菌分离株进行药敏试验。在152株分离株中，最常观察到对氯霉素耐药（17.8%），其次是四环素（14.5%）、环丙沙星（12.5%）、链霉素（11.8%）和氟苯尼考（11.8%），而7.2%为多重耐药。这是关于大熊猫正常阴道可培养细菌菌群及其分离株药敏模式的首次报告。
