Ma Xiaoping, Li Changcheng, Hou Jiafa, Gu Yu
College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, China.
College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China.
BMC Vet Res. 2017 Nov 21;13(1):344. doi: 10.1186/s12917-017-1231-0.
In order to better understand the possible role of fungi in giant panda reproduction and overall health, it is important to provide a baseline for the normal fungal composition in the reproductive system. Using morphology and internal transcribed spacer (ITS) sequence analysis, we systematically isolated and identified fungal species from the vagina, foreskin, and semen of 21 (11 males and 10 females) healthy giant pandas to understand the normal fungal flora of the genital tracts.
A total of 76 fungal strains were obtained, representing 42 genera and 60 species. Among them 47 fungal strains were obtained from vaginal samples, 24 from foreskins, and 5 from semen samples. Several fungal strains were isolated from more than one sample. More fungal species were isolated from females from males. The predominant genera were Aspergillus, Trichosporon, and Penicillium, followed by Candida, Cladosporium, Sordariomycetes, and Diaporthe. The average number of strains in the female vagina was significantly higher than in the foreskin and semen of male.
A total of 60 fungal species (belonging to 42 genera) were identified in the giant panda's genital tract. Some of the species were commonly shared in both males and females. These findings provide novel information on the fungal community in the reproductive tracts of giant pandas.
为了更好地了解真菌在大熊猫繁殖和整体健康中可能发挥的作用，为生殖系统中正常真菌组成提供基线很重要。我们利用形态学和内转录间隔区（ITS）序列分析，系统地从21只（11只雄性和10只雌性）健康大熊猫的阴道、包皮和精液中分离并鉴定真菌物种，以了解生殖道的正常真菌菌群。
共获得76株真菌菌株，代表42个属和60个种。其中47株真菌菌株来自阴道样本，24株来自包皮，5株来自精液样本。有几株真菌从多个样本中分离得到。从雌性中分离出的真菌种类比雄性多。优势属为曲霉属、毛孢子菌属和青霉属，其次是念珠菌属、枝孢菌属、粪壳菌纲和间座壳属。雌性阴道中的菌株平均数显著高于雄性的包皮和精液中的菌株平均数。
在大熊猫生殖道中鉴定出总共60种真菌（属于42个属）。其中一些物种在雄性和雌性中都有。这些发现为大熊猫生殖道中的真菌群落提供了新信息。
