Pundir Sangya, Martin Maria J, O'Donovan Claire
European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Wellcome Trust Genome Campus, Cambridge, United Kingdom.
Swiss Institute of Bioinformatics, University Medical Center, Geneva, Switzerland.
Curr Protoc Bioinformatics. 2016 Mar 24;53:1.29.1-1.29.15. doi: 10.1002/0471250953.bi0129s53.
The Universal Protein Resource (UniProt) is a comprehensive resource for protein sequence and annotation data (UniProt Consortium, 2015). The UniProt Web site receives ∼400,000 unique visitors per month and is the primary means to access UniProt. Along with various datasets that you can search, UniProt provides three main tools. These are the 'BLAST' tool for sequence similarity searching, the 'Align' tool for multiple sequence alignment, and the 'Retrieve/ID Mapping' tool for using a list of identifiers to retrieve UniProtKB proteins and to convert database identifiers from UniProt to external databases or vice versa. This unit provides three basic protocols, three alternate protocols, and two support protocols for using UniProt tools.
通用蛋白质资源（UniProt）是蛋白质序列和注释数据的综合资源（UniProt联盟，2015年）。UniProt网站每月有大约40万独立访客，是访问UniProt的主要途径。除了各种可搜索的数据集外，UniProt还提供三个主要工具。它们是用于序列相似性搜索的“BLAST”工具、用于多序列比对的“Align”工具以及用于使用标识符列表检索UniProtKB蛋白质并将数据库标识符在UniProt与外部数据库之间进行转换（反之亦然）的“检索/ID映射”工具。本单元提供了使用UniProt工具的三个基本方案、三个替代方案和两个支持方案。
