Hadorn Maik, Boenzli Eva, Hanczyc Martin M
Laboratory for Artificial Biology, Centre for Integrative Biology (CIBIO), University of Trento , 38122 Trento, Italy.
Langmuir. 2016 Apr 19;32(15):3561-6. doi: 10.1021/acs.langmuir.5b04003. Epub 2016 Mar 28.
Modular hybrid structures functionalized to assemble in a controlled manner possess diverse properties necessary for a new generation of complex materials and applications. Here, we functionalized giant unilamellar vesicles and emulsion droplets with biotinylated single-stranded DNA oligonucleotides using streptavidin as an intermediary linker to demonstrate specific and reversible DNA-directed self-assembly into vesicle-droplet hybrid structures. A low molar percentage of PEGylated phospholipids independent of the DNA-based recognition machinery at the supramolecular surface modulated the stability of the system. The reversibility of the aggregation was demonstrated by heating the hybrid structures above the melting temperature of the conjoining double-stranded DNA in the presence of excess biotin. The application of this general assembly control system to diverse multiphase soft materials provides the mechanism to assemble complex modular hybrid systems in a controllable and reversible way, which may provide an advantage where multifunctionality is a target property.
功能化以实现可控组装的模块化混合结构具有新一代复杂材料和应用所需的多种特性。在此，我们使用链霉亲和素作为中间连接体，用生物素化的单链DNA寡核苷酸对巨型单层囊泡和乳液滴进行功能化，以证明特定且可逆的DNA导向的自组装成囊泡-液滴混合结构。超分子表面低摩尔百分比的聚乙二醇化磷脂独立于基于DNA的识别机制，调节了系统的稳定性。通过在过量生物素存在下将混合结构加热到连接双链DNA的解链温度以上，证明了聚集的可逆性。将这种通用的组装控制系统应用于多种多相软材料，提供了以可控和可逆方式组装复杂模块化混合系统的机制，这在多功能性是目标特性的情况下可能具有优势。
