Holler Silvia, Casiraghi Federica, Hanczyc Martin Michael
Cellular Computational and Biology Department, CIBIO, Laboratory for Artificial Biology, University of Trento, Via Sommarive 9, Povo 38123, Italy.
Chemical and Biological Engineering, University of New Mexico, Albuquerque, New Mexico 87106, United States.
ACS Omega. 2024 Dec 6;9(50):49316-49322. doi: 10.1021/acsomega.4c06037. eCollection 2024 Dec 17.
Dynamic soft matter systems composed of functionalized vesicles and liposomes are typically produced and then manipulated through external means, including the addition of exogenous molecules. In biology, natural cells possess greater autonomy, as their internal states are continuously updated, enabling them to effect higher order properties of the system. Therefore, a conceptual and technical gap exists between the natural and artificial systems. We engineered functionalized vesicles to form multicore aggregates capable of self-assembly due to the presence of complementary ssDNA strands. A dynamic process was then triggered through an exogenously triggered on-demand release of an endogenously produced displacer molecule, resulting in multicore aggregate disassembly. This approach explores how internal states of vesicles can affect the external organization, demonstrating a very simple programmable strategy for assembly and then endogenous disassembly. This framework supports the exploration of larger and more complex multicore entities, opening a path toward community behavior and a higher degree of autonomy.
由功能化囊泡和脂质体组成的动态软物质系统通常是先制备出来，然后通过外部手段进行操控，包括添加外源性分子。在生物学中，天然细胞具有更大的自主性，因为它们的内部状态在不断更新，使它们能够实现系统的高阶特性。因此，天然系统和人工系统之间存在概念和技术上的差距。我们设计了功能化囊泡，使其由于互补单链DNA的存在而形成能够自组装的多核聚集体。然后通过外源性触发内源性产生的置换分子的按需释放来触发一个动态过程，导致多核聚集体解体。这种方法探索了囊泡的内部状态如何影响外部组织，展示了一种非常简单的用于组装然后内源性解体的可编程策略。这个框架支持对更大、更复杂的多核实体的探索，为群体行为和更高程度的自主性开辟了一条道路。
