Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Food Funct. 2016 Apr;7(4):2043-51. doi: 10.1039/c5fo01621e.
In this study, we prepared a series of lutein nanodispersions via the solvent displacement method, by using surfactants with different stabilizing mechanisms. The surfactants used include Tween 80 (steric stabilization), sodium dodecyl sulfate (SDS; electrostatic stabilization), sodium caseinate (electrosteric stabilization) and SDS-Tween 80 (electrostatic-steric stabilization). We then characterized the resulting lutein nanodispersions in terms of their particle size, particle size distribution, zeta potential, lutein content, flow behavior, apparent viscosity, transmittance, color, morphological properties and their effects on cell viability and cellular uptake. The type of surfactant used significantly (p < 0.05) affected the physical properties of the nanodispersions, but the chemical properties (lutein content) remained unaffected. Transmission electron microscopy (TEM) images obtained from this study demonstrated that the solvent displacement method was capable of producing lutein nanodispersions containing spherical particles with sizes ranging from 66.20-125.25 nm, depending on the type of surfactant used. SDS and SDS-Tween 80 surfactants negatively affected the viability of the HT-29 cells used in this study. Thus, for the cellular uptake determination, only Tween 80 and sodium caseinate surfactants were used. The cellular uptake of the lutein nanodispersion stabilized by sodium caseinate was higher than that which was stabilized by Tween 80. All things considered, the type of surfactant with different stabilizing mechanisms did produce lutein nanodispersions with different characteristics. These findings would aid in future selection of surfactants in order to produce nanodispersions with desirable properties.
在这项研究中，我们通过溶剂置换法，使用具有不同稳定机制的表面活性剂制备了一系列叶黄素纳米分散体。所使用的表面活性剂包括吐温 80（空间稳定）、十二烷基硫酸钠（SDS；静电稳定）、酪蛋白酸钠（电动稳定）和 SDS-吐温 80（静电-空间稳定）。然后，我们根据粒径、粒径分布、Zeta 电位、叶黄素含量、流动行为、表观粘度、透光率、颜色、形态特性以及对细胞活力和细胞摄取的影响来表征所得的叶黄素纳米分散体。所使用的表面活性剂的类型显著（p<0.05）影响纳米分散体的物理性质，但化学性质（叶黄素含量）不受影响。本研究获得的透射电子显微镜（TEM）图像表明，溶剂置换法能够制备含有粒径范围为 66.20-125.25nm 的球形颗粒的叶黄素纳米分散体，具体取决于所使用的表面活性剂的类型。SDS 和 SDS-吐温 80 表面活性剂对本研究中使用的 HT-29 细胞的活力产生负面影响。因此，对于细胞摄取测定，仅使用吐温 80 和酪蛋白酸钠表面活性剂。由酪蛋白酸钠稳定的叶黄素纳米分散体的细胞摄取高于由吐温 80 稳定的叶黄素纳米分散体。综上所述，具有不同稳定机制的表面活性剂确实会产生具有不同特性的叶黄素纳米分散体。这些发现将有助于未来选择表面活性剂，以生产具有理想性质的纳米分散体。
