Fujimura S, Nakamura T
Oral Microbiol Immunol. 1989 Dec;4(4):227-9. doi: 10.1111/j.1399-302x.1989.tb00257.x.
Protease of Bacteroides gingivalis ATCC 33277 was found in intracellular membrane-free, intracellular membrane-bound and extracellular fractions. The insoluble form of proteases was solubilized with a detergent. The elution patterns of proteases on gel filtration in each fraction were rather different. However, enzymatic properties of the proteases separated by gel filtration were very similar.
牙龈卟啉单胞菌ATCC 33277的蛋白酶存在于细胞内膜无结合、细胞内膜结合和细胞外部分。蛋白酶的不溶性形式用去污剂溶解。各部分蛋白酶在凝胶过滤中的洗脱模式差异较大。然而,通过凝胶过滤分离的蛋白酶的酶学性质非常相似。