Goikoetxea M J, Martínez-Aranguren R, Gamboa P, Garcia B E, Gómez F, Bartra J, Parra A, Alvarado M I, Alonso M I, González E, Terrados S, Moya C, Blanca N, Feo-Brito F, Villalba M, Díaz-Perales A, Sanz M L
J Investig Allergol Clin Immunol. 2016;26(1):31-9.
Component-based diagnosis on multiplex platforms is widely used in food allergy but its clinical performance has not been evaluated in nut allergy.
To assess the diagnostic performance of a commercial protein microarray in the determination of specific IgE (sIgE) in peanut, hazelnut, and walnut allergy.
sIgE was measured in 36 peanut-allergic, 36 hazelnut-allergic, and 44 walnut-allergic patients by ISAC 112, and subsequently, sIgE against available components was determined by ImmunoCAP in patients with negative ISAC results. ImmunoCAP was also used to measure sIgE to Ara h 9, Cora 8, and Jug r 3 in a subgroup of lipid transfer protein (LTP)-sensitized nut-allergic patients (positive skin prick test to LTP-enriched extract). sIgE levels by ImmunoCAP were compared with ISAC ranges.
Most peanut-, hazelnut-, and walnut-allergic patients were sensitized to the corresponding nut LTP (Ara h 9, 66.7%; Cor a 8, 80.5%; Jug r 3, 84% respectively). However, ISAC did not detect sIgE in 33.3% of peanut-allergic patients, 13.9% of hazelnut-allergic patients, or 13.6% of walnut-allergic patients. sIgE determination by ImmunoCAP detected sensitization to Ara h 9, Cor a 8, and Jug r 3 in, respectively, 61.5% of peanut-allergic patients, 60% of hazelnut-allergic patients, and 88.3% of walnut-allergic patients with negative ISAC results. In the subgroup of peach LTP-sensitized patients, Ara h 9 sIgE was detected in more cases by ImmunoCAP than by ISAC (94.4% vs 72.2%, P < .05). Similar rates of Cora 8 and Jug r 3 sensitization were detected by both techniques.
The diagnostic performance of ISAC was adequate for hazelnut and walnut allergy but not for peanut allergy. sIgE sensitivity against Ara h 9 in ISAC needs to be improved.
基于组分的多重平台诊断在食物过敏中广泛应用，但在坚果过敏中的临床性能尚未得到评估。
评估一种商用蛋白质芯片在检测花生、榛子和核桃过敏特异性IgE（sIgE）中的诊断性能。
采用ISAC 112检测36例花生过敏、36例榛子过敏和44例核桃过敏患者的sIgE，随后，对ISAC结果阴性的患者采用ImmunoCAP检测针对可用组分的sIgE。还采用ImmunoCAP检测了一组脂质转移蛋白（LTP）致敏的坚果过敏患者（对富含LTP的提取物皮肤点刺试验阳性）中针对Ara h 9、Cora 8和Jug r 3的sIgE水平。将ImmunoCAP检测的sIgE水平与ISAC范围进行比较。
大多数花生、榛子和核桃过敏患者对相应的坚果LTP致敏（分别为Ara h 9，66.7%；Cor a 8，80.5%；Jug r 3，84%）。然而，ISAC在33.3%的花生过敏患者、13.9%的榛子过敏患者或13.6%的核桃过敏患者中未检测到sIgE。ImmunoCAP检测sIgE在ISAC结果阴性的花生过敏患者、榛子过敏患者和核桃过敏患者中分别检测到对Ara h 9、Cor a 8和Jug r 3致敏的比例为61.5%、60%和88.3%。在桃子LTP致敏患者亚组中，ImmunoCAP检测到Ara h 9 sIgE的病例数多于ISAC（94.4%对72.2%，P <.05）。两种技术检测到的Cora 8和Jug r 3致敏率相似。
ISAC的诊断性能对榛子和核桃过敏足够，但对花生过敏不足。ISAC中针对Ara h 9的sIgE敏感性需要提高。
