Koehler M, Mielzyńska I, Bubała H, Sońta-Jakimczyk D, Guzikowska E, Janik-Moszant A, Wieczorek M
Patol Pol. 1989;40(3):311-23.
From January 1987 to April 1988 six children we studied cytologically with the use of alkaline phosphatase-anti-alkaline phosphatase (APAAP) method and monoclonal antibodies (MoP) in order to establish the diagnosis of Non-Hodgkin Lymphoma. (NHL). Cytologic studies concerned pleural fluid (3 patients) imprints of the lymph node (3 patients), bone marrow smears (2 patients) and cerebrospinal fluid (1 patient). We performed simultaneously routine cytologic and histopathologic studies of the lymph nodes. Antigen T6 (thymocytes) was present in blasts of all patients, which permitted us to classify the blasts as common stage II group according to Reinherz. In all cases we found at least two positive antigen detected by MoP pan T (CD2, CD3, CD5, CD7) in one case--no expression of antigen T3 (CD3) and in two cases no antigen detected by an antibody CD2. Antigen Ia was found in one patient, and weak expression of antigen CALLA (CD10) in one patients. In three patients we showed a simultaneous expression of antigens T4, T8, whereas in two patients they were not observed. One child possessed mature phenotype T4, T6. By using APAAP method with MoP in cytologic studies it was possible to diagnose T-lymphoblastic lymphoma in six children before the results of histopathologic examination of the lymph nodes.
1987年1月至1988年4月，我们对6名儿童进行了细胞学研究，采用碱性磷酸酶-抗碱性磷酸酶（APAAP）法和单克隆抗体（MoP），以明确非霍奇金淋巴瘤（NHL）的诊断。细胞学研究涉及胸水（3例）、淋巴结印片（3例）、骨髓涂片（2例）和脑脊液（1例）。我们同时对淋巴结进行了常规细胞学和组织病理学研究。所有患者的原始细胞中均存在抗原T6（胸腺细胞），这使我们能够根据莱因赫兹的标准将原始细胞分类为常见的II期组。在所有病例中，我们发现至少有两种通过MoP泛T（CD2、CD3、CD5、CD7）检测到的阳性抗原，1例中未检测到抗原T3（CD3），2例中未检测到抗体CD2所识别的抗原。1例患者中发现了抗原Ia，1例患者中发现了抗原CALLA（CD10）的弱表达。3例患者同时表达抗原T4、T8，而2例患者未观察到这种表达。1名儿童具有成熟的T4、T6表型。通过在细胞学研究中使用APAAP法和MoP，在淋巴结组织病理学检查结果出来之前，就有可能诊断出6名儿童的T淋巴母细胞淋巴瘤。
