Das D K, Gupta S K, Ayyagari S, Bambery P K, Datta B N, Datta U
Acta Cytol. 1987 Mar-Apr;31(2):119-24.
Review of the records of 243 cases of cytologically diagnosed non-Hodgkin's lymphomas (NHL) revealed pleural effusions in 21 (8.6%). Cytologic examination of pleural fluid was done in 17 cases, of which 16 were reported as positive. Cytologic examination was supplemented with cytochemical staining (acid phosphatase, alpha naphthyl acetate esterase and periodic-acid-Schiff reactions) and E-rosetting studies in 12 cases. Of the 16 positive cases, 11 were malignant lymphomas consisting of convoluted lymphocytes. Acute lymphatic leukemia of the prothymocytic type (T-ALL) and chronic lymphocytic leukemia of the T-cell type (T-CLL) comprised one case each, and there were three cases of follicular center cell lymphomas, two of the cleaved-cell type and one of the Burkitt-type. Comparison of the cytomorphology of the tumor cells in the pleural effusion with those in fine needle aspiration smears from the solid tumors in 14 cases showed an identical appearance in 13 cases; in one, the Burkitt-type lymphoma, the cells were larger and more pleomorphic in the pleural effusion. This study indicates that the cytologic diagnosis and categorization of NHL of the convoluted-cell type is greatly enhanced by the study of neoplastic lymphocytes in a pleural effusion.
对243例经细胞学诊断的非霍奇金淋巴瘤（NHL）病例记录的回顾显示，21例（8.6%）出现胸腔积液。对17例胸腔积液进行了细胞学检查，其中16例报告为阳性。12例病例的细胞学检查辅以细胞化学染色（酸性磷酸酶、α萘乙酸酯酶和过碘酸希夫反应）和E花环试验。在16例阳性病例中，11例为恶性淋巴瘤，由卷曲淋巴细胞组成。原胸腺细胞型急性淋巴细胞白血病（T-ALL）和T细胞型慢性淋巴细胞白血病（T-CLL）各1例，滤泡中心细胞淋巴瘤3例，其中2例为裂细胞型，1例为伯基特型。对14例病例胸腔积液中肿瘤细胞的细胞形态与实体瘤细针穿刺涂片的细胞形态进行比较，13例显示外观相同；1例伯基特型淋巴瘤中，胸腔积液中的细胞更大且多形性更强。本研究表明，通过对胸腔积液中肿瘤淋巴细胞的研究，可大大提高卷曲细胞型NHL的细胞学诊断和分类水平。
