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硬粒小麦中蔗糖合酶2基因（Sus2）的鉴定与特征分析

Identification and Characterization of the Sucrose Synthase 2 Gene (Sus2) in Durum Wheat.

作者信息

Volpicella Mariateresa, Fanizza Immacolata, Leoni Claudia, Gadaleta Agata, Nigro Domenica, Gattulli Bruno, Mangini Giacomo, Blanco Antonio, Ceci Luigi R

机构信息

Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari "A. Moro" Bari, Italy.

Department of Agricultural and Environmental Sciences, University of Bari "A. Moro" Bari, Italy.

出版信息

Front Plant Sci. 2016 Mar 10;7:266. doi: 10.3389/fpls.2016.00266. eCollection 2016.

DOI:10.3389/fpls.2016.00266
PMID:27014292
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4785679/
Abstract

Sucrose transport is the central system for the allocation of carbon resources in vascular plants. Sucrose synthase (SUS), which reversibly catalyzes sucrose synthesis and cleavage, represents a key enzyme in the control of the flow of carbon into starch biosynthesis. In the present study the genomic identification and characterization of the Sus2-2A and Sus2-2B genes coding for SUS in durum wheat (cultivars Ciccio and Svevo) is reported. The genes were analyzed for their expression in different tissues and at different seed maturation stages, in four tetraploid wheat genotypes (Svevo, Ciccio, Primadur, and 5-BIL42). The activity of the encoded proteins was evaluated by specific activity assays on endosperm extracts and their structure established by modeling approaches. The combined results of sucrose synthase 2 expression and activity levels were then considered in the light of their possible involvement in starch yield.

摘要

蔗糖转运是维管植物中碳资源分配的核心系统。蔗糖合酶（SUS）可逆地催化蔗糖合成与分解，是控制碳流入淀粉生物合成过程中的关键酶。本研究报道了硬粒小麦（品种Ciccio和Svevo）中编码SUS的Sus2 - 2A和Sus2 - 2B基因的基因组鉴定及特征分析。对这两个基因在四种四倍体小麦基因型（Svevo、Ciccio、Primadur和5 - BIL42）的不同组织及不同种子成熟阶段的表达情况进行了分析。通过对胚乳提取物进行比活性测定评估了所编码蛋白质的活性，并采用建模方法确定了其结构。随后，根据蔗糖合酶2的表达和活性水平可能对淀粉产量产生的影响，综合考虑了相关结果。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8a0/4785679/c23370c4f359/fpls-07-00266-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8a0/4785679/748259cea187/fpls-07-00266-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8a0/4785679/a654b7007cee/fpls-07-00266-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8a0/4785679/be1de014917a/fpls-07-00266-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8a0/4785679/ea6852380fc8/fpls-07-00266-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8a0/4785679/c23370c4f359/fpls-07-00266-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8a0/4785679/748259cea187/fpls-07-00266-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8a0/4785679/a654b7007cee/fpls-07-00266-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8a0/4785679/be1de014917a/fpls-07-00266-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8a0/4785679/ea6852380fc8/fpls-07-00266-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a8a0/4785679/c23370c4f359/fpls-07-00266-g005.jpg

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