Mahdavi Poor Behroz, Rashedi Jalil, Asgharzadeh Mohammad, Fallah Esmaeel, Hatam-Nahavandi Kareem, Dalimi Abdolhossein
Department of Laboratory Science, Faculty of Paramedicine, Tabriz University of Medical Sciences, Tabriz, Iran. ; Department of Medical Parasitology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Department of Laboratory Science, Faculty of Paramedicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Int J Mol Cell Med. 2015 Fall;4(4):235-9.
Cryptosporidium is one of the most common causes of childhood diarrhea in developing countries. The aim of this randomized pilot study was to detect and characterize infective species and determine the genotypes of Cryptosporidium parasites in pediatric patients suffering from diarrhea in North West of Iran. A total of 113 fecal samples were collected from diarrheic children hospitalized in Tabriz Pediatric Hospital. The amplification of small subunit ribosomal RNA gene was performed using a nested polymerase chain reaction protocol and its products were digested using two restriction enzymes for Cryptosporidium species and genotype differentiation. Cryptosporidium oocysts were found in 2 (1.76%) children with diarrhea and restriction pattern revealed the presence of C.parvum bovine genotype in both positive fecal samples. The findings indicate that Cryptosporidium parvum is responsible for cryptosporidiosis in children in the study region and probably zoonotic transmission is the predominant route of parasite transmission.
隐孢子虫是发展中国家儿童腹泻最常见的病因之一。这项随机试点研究的目的是检测和鉴定感染性物种，并确定伊朗西北部腹泻儿科患者中隐孢子虫寄生虫的基因型。总共从大不里士儿童医院住院的腹泻儿童中收集了113份粪便样本。使用巢式聚合酶链反应方案对小亚基核糖体RNA基因进行扩增，其产物用两种限制性内切酶消化以区分隐孢子虫物种和基因型。在2名（1.76%）腹泻儿童中发现了隐孢子虫卵囊，限制性图谱显示两份阳性粪便样本中均存在牛基因型微小隐孢子虫。研究结果表明，微小隐孢子虫是研究区域儿童隐孢子虫病的病因，人畜共患传播可能是该寄生虫传播的主要途径。
