Chen Fengming, Lin Luyao, Zhang Jie, He Ziyi, Uchiyama Katsumi, Lin Jin-Ming
Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University , Beijing 100084, China.
Department of Applied Chemistry, Graduate School of Urban Environmental Sciences, Tokyo Metropolitan University , Minamiohsawa, Hachioji, Tokyo 192-0397, Japan.
Anal Chem. 2016 Apr 19;88(8):4354-60. doi: 10.1021/acs.analchem.5b04749. Epub 2016 Apr 4.
This study describes a novel method for single-cell analysis and lipid profiling by combining drop-on-demand inkjet cell printing and probe electrospray ionization mass spectrometry (PESI-MS). Through inkjet sampling of a cell suspension, droplets with single cells were generated, precisely dripped onto a tungsten-made electrospray ionization needle, and immediately sprayed under a high-voltage electric field. Lipid fingerprints of single cells were obtained by a mass spectrometry (MS) detector. A homemade magnetic stirring device was applied to the cell suspension reservoir, which controlled the homogeneous distribution of cells in liquid and improved the single-cell-droplet percentage by 43.8%. Eight types of single cells were screened in our platform and further differentiated by principal component analysis based on cellular surface phospholipids. Thus, this study successfully provides a facile method for the direct MS profiling of single-cell lipids by PESI-MS.
本研究描述了一种通过结合按需滴液喷墨细胞打印和探针电喷雾电离质谱（PESI-MS）进行单细胞分析和脂质谱分析的新方法。通过对细胞悬液进行喷墨采样，生成含有单个细胞的液滴，精确滴落在钨制电喷雾电离针上，并在高压电场下立即进行喷雾。通过质谱（MS）检测器获得单细胞的脂质指纹图谱。在细胞悬液储液器中应用了自制的磁力搅拌装置，该装置控制细胞在液体中的均匀分布，并将单细胞液滴百分比提高了43.8%。在我们的平台上筛选了八种类型的单细胞，并基于细胞表面磷脂通过主成分分析进一步区分。因此，本研究成功地提供了一种通过PESI-MS直接对单细胞脂质进行质谱分析的简便方法。
