Sharbatkhari Mahrokh, Shobbar Zahra-Sadat, Galeshi Serrolah, Nakhoda Babak
Molecular Physiology Department, Agricultural Biotechnology Research Institute of Iran (ABRII), AREEO, 3135933151, Karaj, Iran.
Department of Crop Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
Planta. 2016 Jul;244(1):191-202. doi: 10.1007/s00425-016-2497-3. Epub 2016 Mar 25.
Fructan accumulation and remobilization to grains under salinity can decrease dependency of the wheat tolerant cultivar on current photosynthesis and protect it from severe yield loss under salt stress. Tolerance of plants to abiotic stresses can be enhanced by accumulation of soluble sugars, such as fructan. The current research sheds light on the role of stem fructan remobilization on yield of bread wheat under salt stress conditions. Fructan accumulation and remobilization as well as relative expression of the major genes of fructan metabolism were investigated in the penultimate internodes of 'Bam' as the salt-tolerant and 'Ghods' as the salt-sensitive wheat cultivars under salt-stressed and controlled conditions and their correlations were analyzed. More fructan production and higher efficiency of fructan remobilization was detected in Bam cultivar under salinity. Up-regulation of sucrose: sucrose 1-fructosyltransferase (1-SST) and sucrose: fructan 6-fructosyltransferase (6-SFT) (fructan biosynthesis genes) at anthesis and up-regulation of fructan exohydrolase (1-FEH) and vacuolar invertase (IVR) genes (contributed to fructan metabolism) during grain filling stage and higher expression of sucrose transporter gene (SUT1) in Bam was in accordance with its induced fructan accumulation and remobilization under salt stress. A significant correlation was observed between weight density, WSCs and gene expression changes under salt stress. Based on the these results, increased fructan production and induced stem reserves remobilization under salinity can decrease dependency of the wheat tolerant cultivar on current photosynthesis and protect it from severe yield loss under salt stress conditions.
在盐分胁迫下，果聚糖的积累及其向籽粒的再转运可降低耐盐小麦品种对当前光合作用的依赖，并保护其免受盐胁迫下的严重产量损失。植物对非生物胁迫的耐受性可通过积累可溶性糖（如 果聚糖）来增强。当前的研究揭示了茎中果聚糖再转运在盐胁迫条件下面包小麦产量中的作用。研究了耐盐小麦品种“Bam”和盐敏感小麦品种“Ghods”在盐分胁迫和对照条件下，倒数第二节间中果聚糖的积累、再转运以及果聚糖代谢主要基因的相对表达，并分析了它们之间的相关性。在盐分胁迫下，Bam 品种检测到更多的果聚糖产生和更高的果聚糖再转运效率。在开花期，蔗糖：蔗糖 1-果糖基转移酶（1-SST）和蔗糖：果聚糖 6-果糖基转移酶（6-SFT）（果聚糖生物合成基因）上调，在灌浆期，果聚糖外水解酶（1-FEH）和液泡转化酶（IVR）基因（参与果聚糖代谢）上调，并且 Bam 中蔗糖转运蛋白基因（SUT1）的较高表达与其在盐胁迫下诱导的果聚糖积累和再转运一致。在盐胁迫下，重量密度、水溶性碳水化合物（WSCs）和基因表达变化之间观察到显著相关性。基于这些结果，在盐分胁迫下增加果聚糖产量并诱导茎储备再转运可降低耐盐小麦品种对当前光合作用的依赖，并保护其免受盐胁迫条件下的严重产量损失。
