Raj Abhishek, Nachiappan Vasanthi
Biomembrane Lab, Department of Biochemistry, School of Life Sciences, Bharathidasan University, Tiruchirappalli, Tamilnadu, 620024, India.
Antonie Van Leeuwenhoek. 2016 Jun;109(6):841-54. doi: 10.1007/s10482-016-0684-7. Epub 2016 Mar 25.
Hydroquinone (HQ) and benzoquinone (BQ) are known benzene metabolites that form reactive intermediates such as reactive oxygen species (ROS). This study attempts to understand the effect of benzene metabolites (HQ and BQ) on the antioxidant status, cell morphology, ROS levels and lipid alterations in the yeast Saccharomyces cerevisiae. There was a reduction in the growth pattern of wild-type cells exposed to HQ/BQ. Exposure of yeast cells to benzene metabolites increased the activity of the anti-oxidant enzymes catalase, superoxide dismutase and glutathione peroxidase but lead to a decrease in ascorbic acid and reduced glutathione. Increased triglyceride level and decreased phospholipid levels were observed with exposure to HQ and BQ. These results suggest that the enzymatic antioxidants were increased and are involved in the protection against macromolecular damage during oxidative stress; presumptively, these enzymes are essential for scavenging the pro-oxidant effects of benzene metabolites.
对苯二酚（HQ）和苯醌（BQ）是已知的苯代谢产物，它们会形成活性中间体，如活性氧（ROS）。本研究旨在了解苯代谢产物（HQ和BQ）对酿酒酵母抗氧化状态、细胞形态、ROS水平和脂质变化的影响。暴露于HQ/BQ的野生型细胞的生长模式有所下降。酵母细胞暴露于苯代谢产物会增加抗氧化酶过氧化氢酶、超氧化物歧化酶和谷胱甘肽过氧化物酶的活性，但会导致抗坏血酸和还原型谷胱甘肽减少。暴露于HQ和BQ会观察到甘油三酯水平升高和磷脂水平降低。这些结果表明，酶促抗氧化剂增加，并参与了氧化应激期间对大分子损伤的保护；据推测，这些酶对于清除苯代谢产物的促氧化作用至关重要。
