Shalili Amirreza, Mahabadi Hassan Assilian, Variani Ali Safari
Department of Occupational Health and Safety Engineering, Faculty of Medical Science, University of Tarbiat Modarres, Tehran, Iran.
Department of Occupational Health and Safety, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Toxicol Rep. 2024 Dec 4;13:101849. doi: 10.1016/j.toxrep.2024.101849. eCollection 2024 Dec.
Occupational exposures are generally complex, workers are exposed with more than one hazardous agent in work environment. Combined exposure to noise and benzene is common in occupational environments. Sub-acute exposure to benzene vapors can induce oxidative stress in serum. Additionally, noise exposure leads to non-auditory effects, such as oxidative stress. However, In the authors' research scope, a study aimed at investigating the effect of co-exposure to noise and benzene on the oxidative stress of rat serum has not been found. The purpose of this study is to investigate the effect of co-exposure to noise and benzene on oxidative stress. In this study, 24 Wistar albino male rats were divided into four groups: the control group (1), the group exposed to white noise with an intensity of 100 dB and a frequency of 250-8KHz (2), the group exposed to benzene vapors with a concentration of 300 PPM (3), and the group co-exposed to white noise with an intensity of 100 dB and benzene vapors with a concentration of 300 PPM. Oxidative stress induced was investigated by serum oxidative stress indices, including lipid peroxidation (MDA), total oxidative capacity (TOS), and antioxidant activity indices (SOD) and (GSH), as well as total antioxidant capacity (TAC).The results showed that exposure to noise and benzene, both separately and combined, can lead to oxidative stress in rat serum, increasing serum oxidant indices MDA and TOS while decreasing serum antioxidant indices TAC, SOD, and GSH was detected in groups exposed to noise and benzene. The findings indicate that the serum oxidative stress caused by the co- exposure to noise and benzene is significantly higher than separate exposure to noise or benzene, also Co-exposure to noise and benzene can have an almost additive effect on increasing serum oxidative stress in rats. This study highlights the importance of studying co-exposure to physical and chemical hazardous agents in the work environment.
职业暴露通常较为复杂，工人在工作环境中会接触到不止一种有害因素。在职业环境中，噪声和苯的联合暴露很常见。亚急性接触苯蒸气可诱导血清中的氧化应激。此外，噪声暴露会导致非听觉效应，如氧化应激。然而，在作者的研究范围内，尚未发现有旨在研究噪声和苯联合暴露对大鼠血清氧化应激影响的研究。本研究的目的是调查噪声和苯联合暴露对氧化应激的影响。在本研究中，将24只Wistar白化雄性大鼠分为四组：对照组（1组）、暴露于强度为100 dB、频率为250 - 8KHz的白噪声的组（2组）、暴露于浓度为300 ppm苯蒸气的组（3组）以及暴露于强度为100 dB的白噪声和浓度为300 ppm苯蒸气的联合暴露组。通过血清氧化应激指标来研究诱导的氧化应激，这些指标包括脂质过氧化（MDA）、总氧化能力（TOS）、抗氧化活性指标（SOD）和（GSH）以及总抗氧化能力（TAC）。结果表明，单独或联合暴露于噪声和苯均可导致大鼠血清氧化应激，在暴露于噪声和苯的组中检测到血清氧化剂指标MDA和TOS升高而血清抗氧化指标TAC、SOD和GSH降低。研究结果表明，噪声和苯联合暴露引起的血清氧化应激显著高于单独暴露于噪声或苯，而且噪声和苯联合暴露对增加大鼠血清氧化应激几乎具有相加作用。本研究强调了研究工作环境中物理和化学有害因素联合暴露的重要性。
