人类N-BAR结构域在塑造肌膜中的超分子组织。
Supramolecular organization of the human N-BAR domain in shaping the sarcolemma membrane.
作者信息
Daum Bertram, Auerswald Andrea, Gruber Tobias, Hause Gerd, Balbach Jochen, Kühlbrandt Werner, Meister Annette
机构信息
Department of Structural Biology, Max Planck Institute of Biophysics, Max-von-Laue Str. 3, 60438 Frankfurt am Main, Germany.
Mitteldeutsches Zentrum für Struktur und Dynamik der Proteine, ZIK HALOmem, Martin Luther University Halle-Wittenberg, 06120 Halle, Germany.
出版信息
J Struct Biol. 2016 Jun;194(3):375-82. doi: 10.1016/j.jsb.2016.03.017. Epub 2016 Mar 22.
The 30kDa N-BAR domain of the human Bin1 protein is essential for the generation of skeletal muscle T-tubules. By electron cryo-microscopy and electron cryo-tomography with a direct electron detector, we found that Bin1-N-BAR domains assemble into scaffolds of low long-range order that form flexible membrane tubules. The diameter of the tubules closely matches the curved shape of the N-BAR domain, which depends on the composition of the target membrane. These insights are fundamental to our understanding of T-tubule formation and function in human skeletal muscle.
人类Bin1蛋白的30kDa N-BAR结构域对于骨骼肌T小管的形成至关重要。通过使用直接电子探测器的电子冷冻显微镜和电子冷冻断层扫描技术，我们发现Bin1-N-BAR结构域组装成低长程有序的支架，形成柔性膜小管。小管的直径与N-BAR结构域的弯曲形状紧密匹配，这取决于靶膜的组成。这些见解对于我们理解人类骨骼肌中T小管的形成和功能至关重要。
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