Custódio Catarina A, Reis Rui L, Mano João F
3B's Research Group - Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence of Tissue Engineering and Regenerative Medicine , Avepark - Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco GMR, Portugal.
ICVS/3B's PT Government Associated Laboratory , Braga/Guimarães, Portugal.
Biomacromolecules. 2016 May 9;17(5):1602-9. doi: 10.1021/acs.biomac.5b01736. Epub 2016 Apr 7.
Laminarin is a low-molecular-weight (<10 kDa) glucan found in brown algae made up of β(1→3)-glucan with β(1→6)-branches. This is one of the most abundant carbon sources in the marine ecosystem. Laminarin has been found to possess various biological interesting properties, such as antioxidant and antimicrobial activities. An attractive feature of laminarin is its inherently low viscosity and high solubility in organic and aqueous solvents that facilitate processing. This makes laminarin an appealing material for the development of new hydrogels that can be easily injected through minimally invasive procedures or used for microfabrication of hydrogels. An approach for synthesizing photo-cross-linkable laminarin hydrogels is presented in this work for the first time. Photo-cross-linkable laminarin was prepared by chemical modification with acrylate groups. The synthesized photo-cross-linkable laminarin material provides the basis for the development of a new injectable system for biomedical purposes that could be used alone or with encapsulated cells or biological molecules. The cross-linking of the methacrylated laminarin is straightforward via photoinitiated polymerization. The possibility to control the methacrylation degree of laminarin and to prepare solutions up to at least 15% w/v permits us to obtain hydrogels with tuned and wide range of stiffness and swelling. Furthermore, the encapsulation of human-adipose-derived stem cells encapsulated in the photo-cross-linked hydrogels demonstrated in vitro biocompatibility.
海带多糖是一种在褐藻中发现的低分子量（<10 kDa）葡聚糖，由带有β(1→6)分支的β(1→3)-葡聚糖组成。它是海洋生态系统中最丰富的碳源之一。已发现海带多糖具有多种有趣的生物学特性，如抗氧化和抗菌活性。海带多糖的一个吸引人的特点是其固有低粘度以及在有机和水性溶剂中的高溶解性，这有利于加工。这使得海带多糖成为开发新型水凝胶的有吸引力的材料，这种水凝胶可以通过微创手术轻松注射或用于水凝胶的微加工。本文首次提出了一种合成可光交联海带多糖水凝胶的方法。通过用丙烯酸酯基团进行化学修饰制备了可光交联海带多糖。合成的可光交联海带多糖材料为开发一种新型生物医学用可注射系统提供了基础，该系统可单独使用或与封装的细胞或生物分子一起使用。甲基丙烯酸化海带多糖的交联通过光引发聚合直接进行。控制海带多糖甲基丙烯酸化程度并制备浓度至少为15% w/v的溶液的可能性，使我们能够获得具有可调且范围广泛的硬度和溶胀度的水凝胶。此外，封装在光交联水凝胶中的人脂肪来源干细胞的封装证明了其体外生物相容性。
