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光交联的甲基丙烯酰化丝素蛋白和甲基丙烯酰化透明质酸复合水凝胶的制备及表征。

Preparation and Characterization of Photo-Cross-Linkable Methacrylated Silk Fibroin and Methacrylated Hyaluronic Acid Composite Hydrogels.

机构信息

Department of Pharmaceutical Chemistry, Riga Stradins University, Riga LV-1007, Latvia.

Baltic Biomaterials Centre of Excellence, Headquarters at Riga Technical University, Riga LV-1048, Latvia.

出版信息

Biomacromolecules. 2024 Nov 11;25(11):7078-7097. doi: 10.1021/acs.biomac.4c00319. Epub 2024 Oct 14.

DOI:10.1021/acs.biomac.4c00319
PMID:39401165
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11558566/
Abstract

Composite biomaterials with excellent biocompatibility and biodegradability are crucial in tissue engineering. In this work, a composite protein and polysaccharide photo-cross-linkable hydrogel was prepared using silk fibroin methacrylate (SFMA) and hyaluronic acid methacrylate (HAMA). SFMA was obtained by the methacrylation of degummed SF with glycidyl methacrylate (GMA), while HA was methacrylated by 2-aminoethyl methacrylate hydrochloride (AEMA). We investigated the effect of the addition of 1 wt % HAMA to 5, 10, and 20 wt % SFMA, which resulted in an increase in both static and cycling mechanical strengths. All composite hydrogels gelled under UV light in <30 s, allowing for rapid stabilization and stiffness increases. The biocompatibility of the hydrogels was confirmed by direct and indirect contact methods and by evaluation against the NIH3T3 and MC3T3 cell lines with a live-dead assay by confocal imaging. The range of obtained mechanical properties from developed composite and UV-cross-linkable hydrogels sets the basis as possible future biomaterials for various biomedical applications.

摘要

具有优异的生物相容性和可生物降解性的复合材料在组织工程中至关重要。在这项工作中，使用丝素甲酰（SFMA）和透明质酸甲酰（HAMA）制备了一种复合蛋白质和多糖光交联水凝胶。SFMA 是通过甘醇甲基丙烯酸酯（GMA）对脱胶 SF 的甲酰化获得的，而 HA 是通过 2-氨基乙基甲基丙烯酸盐酸盐（AEMA）甲酰化的。我们研究了向 5wt%、10wt%和 20wt%SFMA 中添加 1wt%HAMA 的影响，这导致静态和循环机械强度都增加。所有复合水凝胶在<30s 内通过紫外光交联，允许快速稳定和增加刚度。通过直接和间接接触方法以及通过与 NIH3T3 和 MC3T3 细胞系的活死检测对水凝胶的生物相容性进行评估，并用共焦成像进行评估，证实了水凝胶的生物相容性。所获得的机械性能范围为开发的复合和紫外交联水凝胶奠定了基础，可能成为各种生物医学应用的未来生物材料。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3709/11558566/22303386c395/bm4c00319_0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3709/11558566/328417534bc3/bm4c00319_0001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3709/11558566/086fd58131a4/bm4c00319_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3709/11558566/57178cac20d2/bm4c00319_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3709/11558566/2ca0fce95a92/bm4c00319_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3709/11558566/c444abdfd8d4/bm4c00319_0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3709/11558566/22303386c395/bm4c00319_0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3709/11558566/328417534bc3/bm4c00319_0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3709/11558566/c836ec68153f/bm4c00319_0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3709/11558566/439e67f3974f/bm4c00319_0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3709/11558566/086fd58131a4/bm4c00319_0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3709/11558566/57178cac20d2/bm4c00319_0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3709/11558566/2ca0fce95a92/bm4c00319_0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3709/11558566/c444abdfd8d4/bm4c00319_0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3709/11558566/22303386c395/bm4c00319_0008.jpg

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