Moghtader Farzaneh, Eğri Sinan, Piskin Erhan
a Division of Nanotechnology and Nanomedicine , Institute of Graduate Studies, Hacettepe University , Ankara , Turkey.
b Nanobiyomedtek , Ankara , Turkey.
Artif Cells Nanomed Biotechnol. 2017 Mar;45(2):357-363. doi: 10.3109/21691401.2016.1153485. Epub 2016 Mar 26.
In order to increase stabilities and controlled/sustained released of T4 phages were encapsulated within alginate beads which were then coated with chitosan, polyethylene imine (PEI). Quite high loading capacities (over 90%) were achieved in these pH-sensitive microbeads. Coating with those polycations increased significantly stability both in "simulated gastric fluid" and bile salts especially in the case of PEI coating. The tests conducted in "simulated intestinal fluid" demonstrated that phages were released from the beads which were active at basic pH in which the release rates were smaller in case of chitosan. PEI concluded to be a better coating then chitosan.
为了提高T4噬菌体的稳定性并实现其控释/缓释，将噬菌体包裹在藻酸盐珠中，然后用壳聚糖、聚乙烯亚胺（PEI）进行包衣。在这些pH敏感的微珠中实现了相当高的载量（超过90%）。用这些聚阳离子进行包衣显著提高了在“模拟胃液”和胆盐中的稳定性，尤其是PEI包衣的情况。在“模拟肠液”中进行的测试表明，噬菌体从珠子中释放出来，在碱性pH条件下具有活性，壳聚糖包衣的珠子释放速率较小。结果表明，PEI是比壳聚糖更好的包衣材料。
