Laboratory of Microbiology and Parasitology, School of Pharmacy, Autonomous University of the State of Morelos, 1001 University Avenue, Chamilpa, Cuernavaca 62209, Mexico.
Departament of Medicine, McMaster University, 1280 Main Street West, Hamilton, ON L8S 4K1, Canada.
Viruses. 2021 Sep 25;13(10):1932. doi: 10.3390/v13101932.
Modern bacteriophage encapsulation methods based on polymers such as alginate have been developed recently for their use in phage therapy for veterinary purposes. In birds, it has been proven that using this delivery system allows the release of the bacteriophage in the small intestine, the site of infection by spp. This work designed an approach for phage therapy using encapsulation by ionotropic gelation of the lytic bacteriophage S1 for in 2% / alginate beads using 2% / calcium chloride as crosslinking agent. This formulation resulted in beads with an average size of 3.73 ± 0.04 mm and an encapsulation efficiency of 70%. In vitro, the beads protected the bacteriophages from pH 3 and released them at higher pH. To confirm that this would protect the bacteriophages from gastrointestinal pH changes, we tested the phage infectivity in vivo assay. Using a model chicken () infected with Enteritidis, we confirmed that after 3 h of the beads delivery, infective phages were present in the chicken's duodenal and caecal sections. This study demonstrates that our phage formulation is an effective system for release and delivery of bacteriophage S1 against Enteritidis with potential use in the poultry sector.
最近开发了基于聚合物（如海藻酸盐）的现代噬菌体包封方法，用于兽医噬菌体治疗。在鸟类中，已经证明使用这种输送系统可以使噬菌体在感染部位的小肠中释放出来。本研究设计了一种使用离子凝胶化包封裂解噬菌体 S1 治疗 的方法，在 2%/海藻酸钠珠中使用 2%/氯化钙作为交联剂。该制剂得到的珠粒平均粒径为 3.73±0.04mm，包封效率为 70%。体外实验中，珠粒能保护噬菌体免受 pH3 的影响，并在较高 pH 值下释放噬菌体。为了确认这可以保护噬菌体免受胃肠道 pH 值变化的影响，我们进行了体内噬菌体感染性试验。使用感染肠炎沙门氏菌的鸡（）模型，我们证实，在珠粒给药 3 小时后，鸡的十二指肠和盲肠部分存在感染性噬菌体。本研究表明，我们的噬菌体配方是一种有效的释放和递送噬菌体 S1 对抗肠炎沙门氏菌的系统，具有在禽类行业应用的潜力。
